Characterization of the respiratory microbiome and virome associated with bovine respiratory disease complex

Authors: McDaneld, Tara; EARLEY, BERNADETTE - Teagasc (AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY); MCGEE, MARK - Teagasc (AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY); Kuehn, Larry; Workman, Aspen; CONANT, GAVIN - North Carolina State University; CORMICAN, PAUL - Teagasc (AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY); MCCABE, MATTHEW - Teagasc (AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY); DUFFY, CATHERINE - Agri-Food And Biosciences Institute; LEMON, KEN - Agri-Food And Biosciences Institute; MCMENAMY, MICHAEL - Agri-Food And Biosciences Institute; SMYTH, VICTORIA - Agri-Food And Biosciences Institute; Smith, Timothy - Tim; COSBY, S. LOUISE - Agri-Food And Biosciences Institute

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/17/2019
Publication Date: 11/4/2019
Citation: McDaneld, T.G., Earley, B., McGee, M., Kuehn, L.A., Workman, A.M., Conant, G., Cormican, P., McCabe, M., Duffy, C., Lemon, K., McMenamy, M., Smyth, V., Smith, T.P., Cosby, L. 2019. Characterization of the respiratory microbiome and virome associated with bovine respiratory disease complex [abstract]. Conference of Research Workers in Animal Diseases Conference, November 2-5, 2019, Chicago, Illinois. P213. Available: https://crwad.org/crwad-2019/

Interpretive Summary:

Technical Abstract: Objective This project proposal was submitted as a Tri-partite collaborative through NIFA’s Food and Agriculture Research Initiative with Ireland (ROI) and Northern Ireland (NI) for the Animal Health and Disease Program Area Priority (A1221). The proposal is comprised of three inter-related projects, which will each investigate specific research objectives and will benefit from the complementary expertise of principal researchers that are integral to the project. Methods The project will pool research resources available at the US Meat Animal Research Center (US), Teagasc (ROI), and the Agri-Food and Biosciences Institute (NI). Bovine respiratory disease (BRD; pneumonia) is one of the most significant health problems in cattle and the most expensive animal disease afflicting herds in the cattle industry. Effective immunization or antimicrobial therapies that substantially reduce the prevalence or severity of BRD have not been developed despite decades of research, due to the multifactorial etiopathogenesis of the disease that encompasses an array of infectious agents, as well as environmental and management potentiating factors. Conclusions In this multidisciplinary project, we aim to 1) investigate the prevalence and distribution of the respiratory microbiome and virome associated with BRD in beef herds at the US Meat Animal Research Center (USMARC) and in beef and dairy herds in Ireland (Teagasc); 2) employ next-generation sequencing (NGS), third-generation sequencing (TGS), bioinformatic technologies, and high throughput sensitive and rapid diagnostics to identify respiratory viral and bacterial agents associated with BRD (USMARC and Teagasc); and 3) elucidate the dynamics of secondary viral and bacterial infection by monitoring experimentally virus infected animals in longitudinal studies (Agri-Food and Biosciences Institute (AFBI, N. Ireland)).