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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #365248
Research Project: Development of New Production Methodologies for Biocontrol Agents and Fastidious Microbes to Improve Plant Disease Management

Location: Crop Bioprotection Research

Title: Methylobacterium segetis sp. nov., a novel member of the family Methylobacteriaceae isolated from soil on Jeju Island

Author
item TEN, LEONID - Kyungpook National University
item LI, WEILAN - Kyungpook National University
item ELDERINY, NABIL SALAH - Kyungpook National University
item KIM, MYUNG KYUM - Seoul Women'S University
item LEE, SEUNG-YEOL - Kyungpook National University
item Rooney, Alejandro - Alex
item JUNG, HEE-YOUNG - Kyungpook National University

Submitted to: Archives of Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/25/2019
Publication Date: 12/3/2019
Citation: Ten, L.N., Li, W., Elderiny, N., Kim, M., Lee, S., Rooney, A.P., Jung, H. 2020. Methylobacterium segetis sp. nov., a novel member of the family Methylobacteriaceae isolated from soil on Jeju Island. Archives of Microbiology. 202:747-754. https://doi.org/10.1007/s00203-019-01784-z.
DOI: https://doi.org/10.1007/s00203-019-01784-z

Interpretive Summary: This manuscript reports on the characterization of a new species of bacteria, Methylobacterium segetis, which was isolated from soil on Jeju Island (South Korea). The species possesses novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications will be assessed in future studies.

Technical Abstract: A bacterial strain, 17J42-1T, was isolated from a soil sample collected on Jeju Island, Republic of Korea. Colonies grown on R2A agar were pink in color, and cells were Gram-stain negative, short and rod-shaped. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Methylobacterium in the family Methylobacteriaceae, with high levels of 16S rRNA sequence similarity shared with Methylobacterium oxalidis 35aT (98.6%), Methylobacterium jeotgali S2R03-9T (97.5%), and Methylobacterium soli YIM 48816T (97.3%). Cells grew at 15–35 °C, pH 5–9, and in the presence of 0–1.0% NaCl. The genomic G'+'C content was 70.2 mol% based on the whole genome analysis. The predominant respiratory quinone was ubiquinone Q-10, the major fatty acid was C18:1'7c (85.3%), and the major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The phenotypic and chemotaxonomic data support the affiliation of strain 17J42-1T with the genus Methylobacterium. However, the DNA–DNA relatedness between the isolate and its closest phylogenetic neighbors was lower than 38%. The OrthoANI and dDDH values between strain 17J42-1T and the closest type strain Methylobacterium oxalidis NBRC 107715T were calculated to be 85.9% and 30.6%, respectively. The results of 16S rRNA gene sequence similarity analysis, DNA–DNA hybridization analysis, and the observed differentiating phenotypic properties from other closely related taxa clearly indicate that strain 17J42-1T represents a novel species in the genus Methylobacterium, for which the name Methylobacterium segetis sp. nov. is proposed. The type strain is 17J42-1T (='KCTC 62267T'='JCM 33059T).