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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #366032
Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Complete genome sequence of rose virus A, the first carlavirus identified in rose

Author
item DIAZ-LARA, ALFREDO - University Of California, Davis
item Mollov, Dimitre
item GOLINO, DEBORAH - University Of California, Davis
item AL RWAHNIH, MAHER - University Of California, Davis

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/30/2019
Publication Date: 11/11/2019
Citation: Diaz-Lara, A., Mollov, D.S., Golino, D., Al Rwahnih, M. 2019. Complete genome sequence of rose virus A, the first carlavirus identified in rose. Archives of Virology. https://doi.org/10.1007/s00705-019-04460-1.
DOI: https://doi.org/10.1007/s00705-019-04460-1

Interpretive Summary: Roses (Rosa spp.), one of the most popular ornamentals worldwide, are used as both cut flowers and landscape plants. Many viral diseases affect rose production. It is important to detect and eliminate virus infected roses prior to material being propagated and distributed. We report a new virus identified in rose and provisionally named Rose virus A (RVA). The RVA genomic organization and sequence was determined. This study provides information for the proper classification of RVA. The genome sequence facilitates developing reliable detection methods that can be used to screen rose genetic material and develop better viral control measures for trade and production industries.

Technical Abstract: A novel virus was discovered in a Rosa wichuraiana Crep. by high throughput sequencing, and tentatively named rose virus A (RVA). Based on sequence identity and phylogenetic analysis, RVA represents a new member of the genus Carlavirus (family Betaflexiviridae). The genome of RVA is 8,849 nucleotides long excluding a poly(A) tail and comprises six open reading frames (ORFs). Predicted ORFs include replicase, coat protein, nucleic acid binding protein and triple gene block (TGB) arranged as it would be in a typical carlavirus. RVA is the first carlavirus identified in rose and has the highest nucleotide identity to poplar mosaic virus. Reverse transcription PCR-based assays were developed to confirm RVA in the original source and to screen additional rose plants.