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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #366475
Research Project: Non-Antibiotic Strategies to Control Priority Bacterial Infections in Swine

Location: Virus and Prion Research

Title: Complete genome sequence of Escherichia coli antibiotic resistance (AR) isolate bank #0349

Author
item STUART, KEIRA - Animal And Plant Health Inspection Service (APHIS)
item Shore, Sarah
item Nicholson, Tracy

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/31/2019
Publication Date: 11/27/2019
Citation: Stuart, K.L., Shore, S., Nicholson, T.L. 2019. Complete genome sequence of Escherichia coli antibiotic resistance (AR) isolate bank #0349. Microbiology Resource Announcements. 8(48). https://doi.org/10.1128/MRA.01078-19.
DOI: https://doi.org/10.1128/MRA.01078-19

Interpretive Summary: Colistin is an old antibiotic used for treatment of Gram-negative bacteria infections that fell out of favor in human medicine due to toxicity issues. Due to the rise in antimicrobial resistance, it’s currently used as a last line of defense in the treatment of severe bacterial infections. Colistin resistance was generally regarded to be chromosomally mediated and non-transmissible. However, a plasmid-mediated polymyxin resistance gene named mcr-1 has recently been reported. The investigators demonstrated that the plasmid harboring the mcr-1 gene was stable and readily transferable. This plasmid-mediated polymyxin resistance has heightened public health concern due to the potential for rapid horizontal transfer. Here, we report the generation of whole genome sequence data for Escherichia coli AR Bank # 0349 obtained from the Food and Drug Administration and Centers for Disease Control and Prevention Antibiotic Resistance Isolate Bank, which exhibits resistance to colistin due to a plasmid-borne mcr-1 gene. This information can be directly used to develop diagnostic assays and investigate horizontal transfer mechanisms of antibiotic resistant mobile elements. This information is important to public health professionals, scientists, veterinarians, producers, and consumers.

Technical Abstract: The emergence of plasmid-mediated polymyxin resistance encoded by mcr-1 has heightened public health concerns due to the potential for rapid horizontal transfer. Here, we report the complete genome sequence of Escherichia coli AR Bank # 0349, which exhibits resistance to colistin, encoded by a plasmid-borne mcr-1gene.