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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #366762
Research Project: Nutritional Intervention and Management Strategies to Reduce Stress and Improve Health and Well-being in Cattle and Swine

Location: Livestock Issues Research

Title: Effects of S.c. boulardii CNCMI-1079 on piglets’ response to an inflammation challenge

Author
item Carroll, Jeffery - Jeff Carroll

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2019
Publication Date: 10/23/2019
Citation: Carroll, J.A. 2019. Effects of S.c. boulardii CNCMI-1079 on piglets’ response to an inflammation challenge. Meeting Abstract. p. 110-124. Paris, France, October 23, 2019

Interpretive Summary:

Technical Abstract: To further elucidate a possible “immunological priming” effect of yeast-based products, two studies were conducted to investigate the effects of feeding Saccharomyces cerevisiae boulardii (Scb; CNCM I-1079) to weanling pigs prior to an immune challenge. The first study evaluated the physiological and immunological responses of weaned pigs supplemented with Scb prior to an E. coli-derived lipopolysaccharide (LPS) challenge. Castrated male pigs were assigned to 1 of 2 treatment groups; with (Scb; n = 15) and without (Control; n = 15) the in-feed inclusion of Scb (200 g/ton) for 16 d. On d 16, all pigs were dosed via indwelling jugular catheters with LPS (25 ug/kg BW) at time 0. In Scb-treated pigs, ADG was increased (P < 0.05) by 39.9% and LPS-induced pig mortality was reduced by 20% as compared to Control pigs. White blood cells, lymphocytes and neutrophils were increased (P < 0.05) in Scb-treated pigs prior to LPS dosing compared to Controls. Peak production (P < 0.05) of IL-1beta and IL-6 was decreased in Scb-treated pigs after LPS administration when compared to Controls. Peak TNF-alpha production in Scb-treated pigs was accelerated by 0.5 h and was greater (P < 0.05) than peak production in Controls. The peak production of IFN-gamma was greater and had increased (P < 0.05) amplitude persistent for 3 h in Scb-treated pigs when compared to Controls. The second study evaluated the effect of supplementing the diets of weanling pigs (26 ± 0.2 d) with Scb on performance and tissue content of Salmonella following a dual challenge with LPS and Salmonella. Castrated male pigs were assigned to 1 of 2 treatments (n=10 per treatment): 1) Control, fed a non-medicated commercial starter diet or 2) Scb, fed the non-medicated commercial diet supplemented with Scb (200 g/ton). On d 20, pigs were administered LPS (25 µg/kg BW) i.v. at 0 hr. At 3 h, pigs were orally dosed with Salmonella typhimurium (10 exp 6 CFU/pig). On d 24, pigs were euthanized, and intestinal and lymph tissue contents were collected. Both pre- and post-challenge intraperitoneal temperatures were lower in Scb pigs compared to Control pigs. Additionally, tissue content of Salmonella was reduced in the rectal (P < 0.005) and lymph (P < 0.06) tissues from Scb pigs compared to Control pigs. Collectively, these studies demonstrate that supplementation with Scb can potentially “prime” the immune system of newly weaned pigs, thus improving the immune response to a pathogenic challenge.