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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Endemic Poultry Viral Diseases Research » Research » Publications at this Location » Publication #367174
Research Project: Enhancing Genetic Resistance to Marek’s Disease in Poultry

Location: Endemic Poultry Viral Diseases Research

Title: ALV-miRNA-p19-01 promotes viral replication via targeting dual specificity Phosphatase 6

Author
item YAN, YIMING - South China Agricultural University
item CHEN, SHENG - South China Agricultural University
item LIAO, SHUANG - South China Agricultural Univerisity
item GAO, SHUANG - Guangdong Academy Of Agricultural Sciences
item PANG, YANLING - South China Agricultural Univerisity
item ZHANG, XINHENG - South China Agricultural University
item Zhang, Huanmin
item XIE, QINGMEI - South China Agricultural University

Submitted to: Viruses
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2022
Publication Date: 4/13/2022
Citation: Yan, Y., Chen, S., Liao, S., Gao, S., Pang, Y., Zhang, X., Zhang, H., Xie, Q. 2022. ALV-miRNA-p19-01 promotes viral replication via targeting dual specificity Phosphatase 6. Viruses. https://doi.org/10.3390/v14040805.
DOI: https://doi.org/10.3390/v14040805

Interpretive Summary: MicroRNAs are a small classes of RNA molecules, differing from relatively large RNAs, that do not produce any protein products. But microRNAs are richly documented in regulatory rolls controlling gene expression and large RNA products. One such microRNA was identified in this study, which was originated from one subgroup of avian leukosis virus. Further laboratory examinations showed this microRNA provides functional support in avian leukosis virus infection process. This is the first microRNA identified in this particular subgroup of avian leukosis virus with detected function, which is of importance in better understanding the virus infection mechanism.

Technical Abstract: MicroRNAs (miRNAs) are a group of regulatory noncoding RNAs, serving as major regulators with a sequence-specific manner in multifarious biological processes. Although a series of viral families have been proved to encode miRNAs, few reports were available regarding the function of ALV-J-encoded miRNA. Here, we reported a novel miRNA (designated ALV-miRNA-p19-01) in ALV-J-infected DF-1 cells. We found that ALV-miRNA-p19-01 is encoded by the genome of the ALV-J SCAU1903 strain (located at nucleotides site 779 to 801) in a classic miRNA biogenesis manner. The transfection of DF-1 cells with ALV-miRNA-p19-01 enhanced ALV-J replication, while the blockage of ALV-miRNA-p19-01 suppressed ALV-J replication. Furthermore, our data showed that ALV-miRNAp19-01 promotes ALV-J replication by directly targeting the cellular gene dual specificity phosphatase 6 through regulating ERK2 activity.