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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Publications at this Location » Publication #367428
Research Project: Conservation, Characterization, Evaluation, and Distribution of Grain, Oilseed, Vegetable, Subtropical and Tropical Legume, and Warm Season Grass Genetic Resources and Associated Information

Location: Plant Genetic Resources Conservation Unit

Title: Identifying chromosomal introgressions from a wild species Arachis diogoi into interspecific peanut hybrids

Author
item HANSON, ERIK - University Of Florida
item ZHOU, HAI - University Of Florida
item Tallury, Shyamalrau - Shyam
item YANG, XIPING - University Of Florida
item PAUDEL, DEV - University Of Florida
item TILLMAN, BARRY - University Of Florida
item WANG, JIANPING - University Of Florida

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/10/2020
Publication Date: 5/12/2020
Citation: Hanson, E., Zhou, H., Tallury, S.P., Yang, X., Paudel, D., Tillman, B., Wang, J. 2020. Identifying chromosomal introgressions from a wild species Arachis diogoi into interspecific peanut hybrids. Plant Breeding. https://doi.org/10.1111/pbr.12828.
DOI: https://doi.org/10.1111/pbr.12828

Interpretive Summary: An peanut hybrid population was produced from a cross between Arachis diogoi, a wild species with resistance to Tomato Spotted Wilt Virus (TSWV), and the susceptible Virginia-type cultivar, Gregory. The objectives of the study are to identify genetic material of A. diogoi in the hybrids and to evaluate the spotted wilt resistance in the population. Sixty-three hybrid lines were genotyped using 137 DNA markers, of which 14 markers detected the genetic material of A. diogoi in them. Additionally, more advanced genotyping by sequencing of a subset of the population revealed A. diogoi genetic material in all hybrid lines of the population. Many of these hybrid lines showed consistent spotted wilt resistance, suggesting successful transfer of TSWV resistance.

Technical Abstract: An interspecific peanut hybrid population was generated from a cross between Arachis diogoi, a wild species with resistance to spotted wilt, and a susceptible cultivar Gregory. The objectives of the study are to identify successful introgression of A. diogoi's genome and to evaluate the spotted wilt resistance in the introgression population. Sixty-three putative introgression lines were genotyped using 137 polymorphic simple sequence repeat (SSR) marker, out of which 14 locating on seven of the 20 peanut chromosomes showed introgression of A. diogoi genome into at least one of the introgression lines. In addition, genotyping by sequencing of a subset of the population revealed SNP loci displaying introgression of A. diogoi genome on every chromosome of the A sub-genome of the introgression lines, with the most introgressed loci on chromosome A05. Several introgression lines showed consistent spotted wilt resistance, suggesting successful genomic introgression of A. diogoi contributing to the resistance.