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ARS Home » Midwest Area » Ames, Iowa » National Laboratory for Agriculture and The Environment » Agroecosystems Management Research » Research » Publications at this Location » Publication #367941
Research Project: Reducing Production Losses due to Oxidative Stress and Bacterial Pathogens in Swine

Location: Agroecosystems Management Research

Title: Complete genome sequence of multidrug-resistant Salmonella enterica serovar I 4,[5],12:i:- 2015 U.S. pork outbreak isolate USDA15WA-1

Author
item Bearson, Bradley - Brad
item Trachsel, Julian
item HOLMAN, DEVIN - Orise Fellow
item Brunelle, Brian
item SIVASANKARAN, SATHESH - Iowa State University
item SIMMONS, MUSTAFA - Food Safety Inspection Service (FSIS)
item WASILENKO, JAMIE - Food Safety Inspection Service (FSIS)
item TILLMAN, GLENN - Food Safety Inspection Service (FSIS)
item JOHNSTON, JOHN - Food Safety Inspection Service (FSIS)
item Bearson, Shawn

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/16/2019
Publication Date: 10/3/2019
Citation: Bearson, B.L., Trachsel, J.M., Holman, D.B., Brunelle, B.W., Sivasankaran, S.K., Simmons, M., Wasilenko, J., Tillman, G., Johnston, J.J., Bearson, S.M. 2019. Complete genome sequence of multidrug-resistant Salmonella enterica serovar I 4,[5],12:i:- 2015 U.S. pork outbreak isolate USDA15WA-1. Microbiology Resource Announcements. 8(40):e00791-19. https://doi.org/10.1128/MRA.00791-19.
DOI: https://doi.org/10.1128/MRA.00791-19

Interpretive Summary: Non-typhoidal Salmonella isolates are a leading cause of bacterial foodborne-illness in humans. In 2015, a multistate outbreak of multidrug-resistant (MDR) Salmonella enterica serovar I 4,[5],12:i:- was associated with pork products from Washington state. Illness was confirmed in 188 individuals including 30 hospitalizations and 523,380 pounds of pork were recalled. Genetic information is needed concerning the outbreak strain to determine differences between the outbreak strain and other isolates of Salmonella. The genome sequence of an outbreak-associated strain was determined and analyzed. Two large genetic insertions were found and include a module encoding resistance to four different antimicrobials and a metal tolerance island to copper, silver, and arsenic. The DNA sequence has been archived in a publicly accessible repository. This research data provides genomic information for comparison against databases containing DNA sequences for other Salmonella strains to allow identification and characterization of unique attributes of the pork outbreak-associated isolate.

Technical Abstract: The genome of a multidrug-resistant (MDR) Salmonella enterica subsp. enterica serovar I 4,[5],12:i:- isolate from the 2015 U.S. pork outbreak was sequenced and annotated. The complete nucleotide sequence of USDA15WA-1 is 5,031,277 bp and includes an MDR module inserted in the fljB region and Salmonella Genomic Island 4 encoding tolerance to multiple metals.