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ARS Home » Midwest Area » Bowling Green, Kentucky » Food Animal Environmental Systems Research » Research » Publications at this Location » Publication #368224
Research Project: Developing Safe, Efficient and Environmentally Sound Management Practices for the Use of Animal Manure

Location: Food Animal Environmental Systems Research

Title: PFGE XbaI® Indistinguishable properties of Salmonella Kastrup and Salmonella Larochelle isolates at beef processing and distribution continuum

Author
item HIKO, ADEM - Ethiopia Haramaya University
item Agga, Getahun
item BRAUTIGAM, LIESELOTTE - Freie University
item IRSIGLER, HERLINDE - Freie University
item AMENI, GOBENA - Addis Ababa University
item REINHARD, FRIES - Freie University

Submitted to: Ethiopian Veterinary Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/10/2019
Publication Date: 9/18/2019
Citation: Hiko, A., Agga, G.E., Brautigam, L., Irsigler, H., Ameni, G., Reinhard, F. 2019. PFGE XbaI® Indistinguishable properties of Salmonella Kastrup and Salmonella Larochelle isolates at beef processing and distribution continuum. Ethiopian Veterinary Journal. 23(2):1-12.

Interpretive Summary: Salmonella is a leading cause of foodborne illnesses in humans. Consumption of improperly cooked meat contaminated with Salmonella is a significant source of human infections. Monitoring Salmonella across meat processing and distribution helps to take corrective actions to reduce risk to the public. Various samples at beef cattle processing plant and retail shops were collected, cultured and the isolates were characterized for drug resistance and genetic relatedness. Fourteen Salmonella isolates belonging to Salmonella Kastrup and Salmonella Larochelle were detected from abattoir, cattle and retail beef samples. Over 70% of the isolates had reduced susceptibility to neomycin, 21% were resistant to tetracycline, a single isolate was resistant to gentamicin. Two distinct groups were identified with the first group consisting of 13 isolates and the second group consisting a single Salmonella Larochelle isolate. Results indicate that the meat hygiene practices at the abattoirs and distributions are not adequate to remove Salmonella since genetically similar strains are disseminated across the beef production-processing-distribution continuum.

Technical Abstract: Salmonellosis is a major cause of foodborne illnesses in humans throughout the world. Pulsed field gel electrophoresis (PFGE) is a commonly used tool for epidemiological survey and source tracking of Salmonella strains isolated from various sources. A total of 237 samples from various sources were collected at abattoir and butcheries in Ethiopia from December 2011 to April 2012. Isolates of Salmonella Kastrup (n = 3) and Salmonella Larochelle (n = 11) were characterized for antimicrobial resistance using disc diffusion and for genotypic profiles using PFGE XbaI®. Salmonella Kastrup and S. Larochelle were isolated from 1.3% and 4.6% of total samples (n = 237) examined. Except for water samples (n = 12) and truck swabs (n=11), all other sample sources were positive for one or more of S. Kastrup and S. Larochelle. Salmonella Kastrup was isolated in Ethiopia for the first time. Salmonella isolates from both Kastrup and Larochelle serotypes showed similar antimicrobial resistance profiles. Two pulsotypes consisting of the same three S. Kastrup and 10 S. Larochelle cluster and second cluster consisting of one S. Larochelle were identified at 1.5% and 1% band positions tolerance by unweighted-pair group method (UPGM) and showed =91% similarity. S. Kastrup and S. Larochelle isolates showed similar antimicrobial resistance profiles with indistinguishable PFGE XbaI® patterns indicating possible clonal spread of the isolates along beef processing and distribution continuum.