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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #369100
Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Complete genomic sequence of tea-oil camellia associated deltapartitivirus, a novel virus from Camellia oleifera

Author
item WU, LIPING - Nanchang University
item DU, TAMENG - Nanchang University
item LIU, HUAWEI - Nanchang University
item PENG, LIANG - Nanchang University
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/10/2019
Publication Date: 10/29/2019
Citation: Wu, L., Du, T., Liu, H., Peng, L., Li, R. 2019. Complete genomic sequence of tea-oil camellia associated deltapartitivirus, a novel virus from Camellia oleifera. Archives of Virology. https://doi.org/10.1007/s00705-019-04429-0.
DOI: https://doi.org/10.1007/s00705-019-04429-0

Interpretive Summary: Tea-oil camellia (Camellia oleifera), native to China, is an evergreen shrub from which seed oil used for seasoning and cooking is extracted. In this study, a new virus was identified from tea-oil camellia by a sensitive sequencing technology. The complete genomic sequence of the virus was determined. Analyses of the genetic information show that it is a novel virus is in the DNA- genome deltapartitivirus group. The virus was detected in five of the forty tea-oil camellia trees tested in this study. The study provides the information necessary to classify the virus, and to help develop detection methods to reduce its impact on tea-oil production. This is also the first report of a virus in tea-oil camellia.

Technical Abstract: Virus sequences which contains the whole genome of three dsRNAs with the size of dsRNA1 (1,712 bp), dsRNA2 (1,504 bp) and dsRNA3 (1,353 bp), were identified from an oil tea plant by high-throughput sequencing analysis. The sequences of the three dsRNAs were re-sequenced. The largest one, dsRNA1, has a single open reading frame (ORF) which encodes a putative 52.7 kDa protein of RNA dependent RNA polymerase. dsRNA2 and dsRNA3 were predicted to encode for two putative capsid proteins of 40.5 kDa and 40.6 kDa, respectively. The virus, which is provisionally named oil tea-associated deltapartitivirus, shares 42-63% nucleotide sequence identities to members of the genus Deltapartitivirus at whole genome level. Phylogenetic tree revealed that the virus should be a new member of the genus Deltapartitivirus.