Registration of RMBHMTUP-C4 a Random Mated Population with alleles from Four Gossypium Species introgressed into Upland Cotton Germplasm

Authors: Jenkins, Johnie; McCarty, Jack; Hayes, Russell - Russ; Saha, Sukumar; STELLY, DAVID - Texas Agrilife

Submitted to: Journal of Plant Registrations
Publication Type: Germplasm Registration
Publication Acceptance Date: 8/31/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary: The breeding genetic base for upland cotton Gossypium hirsutum L. needs more diversity. Use of wild species of Gossypium is one approach that is of interest to breeders. However, interspecific crosses between G. hirsutum and wild tetraploid species or the cultivated tetraploid species G. barbadense L. have not been widely used in breeding because of incompatibility between species causing problems in later generations. We have successfully utilized chromosome substitution lines from four tetraploid Gossypium species to introgress alleles from these species into G. hirsutum. We report a random mated population which has achieved introgression of alleles from four Gossypium tetraploid species into one population. Random Mated Barbadense, Hirsutum, Mustelinum, Tomentosum, Upland Population (RMBHMTUP-C4 Reg. No. PI ) is a population that has been random mated for 5 cycles beginning with the 32 F1’s from crossing 32 chromosome substitution lines with one of five upland cultivars. The resulting population is very diverse and should be a valuable contribution to diversify and broaden the genetic base for upland cotton breeding. Although very diverse, it has characteristics that make it very much like an upland cultivar in appearance and fiber properties. We did not observe any sterility or other breeding problems, as normally observed in conventional interspecific crosses, during the development of this population. We harvested about 2,010 bolls in each of the five cycles of random mating (10,050 bolls). Assuming 25 seed per boll, this amounted to 251,250 hand crossed seed each of which was the result of two opportunities (pollen and egg) for recombination of alleles or 502,500 metaphase pairing of chromosomes each with multiple opportunities for recombination of alleles Thus, we have developed a unique random mated population that has an enormous number of opportunities for assortment and recombination of alleles among four tetraploid Gossypium species.

Technical Abstract: The breeding genetic base for upland cotton Gossypium hirsutum L. needs more diversity. Use of wild species of Gossypium is one approach that is of interest to breeders. However, interspecific crosses between G. hirsutum and wild tetraploid species or the cultivated tetraploid species G. barbadense L. have not been widely used in breeding because of incompatibility between species causing problems in later generations. We have successfully utilized chromosome substitution lines from four tetraploid Gossypium species to introgress alleles from these species into G. hirsutum. We report a random mated population which has achieved introgression of alleles from four Gossypium tetraploid species into one population. Random Mated Barbadense, Hirsutum, Mustelinum, Tomentosum, Upland Population (RMBHMTUP-C4 Reg. No. PI ) is a population that has been random mated for 5 cycles beginning with the 32 F1’s from crossing 32 chromosome substitution lines with one of five upland cultivars. The resulting population is very diverse and should be a valuable contribution to diversify and broaden the genetic base for upland cotton breeding. Although very diverse, it has characteristics that make it very much like an upland cultivar in appearance and fiber properties. We did not observe any sterility or other breeding problems, as normally observed in conventional interspecific crosses, during the development of this population. We harvested about 2,010 bolls in each of the five cycles of random mating (10,050 bolls). Assuming 25 seed per boll, this amounted to 251,250 hand crossed seed each of which was the result of two opportunities (pollen and egg) for recombination of alleles or 502,500 metaphase pairing of chromosomes each with multiple opportunities for recombination of alleles Thus, we have developed a unique random mated population that has an enormous number of opportunities for assortment and recombination of alleles among four tetraploid Gossypium species.