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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #369565
Research Project: Intestinal Microbial Ecology and Metagenomic Strategies to Reduce Antibiotic Resistance and Foodborne Pathogens

Location: Food Safety and Enteric Pathogens Research

Title: Complete genome sequence of Campylobacter jejuni strain NADC 20827 isolated from commercial turkeys

Author
item Sylte, Matthew
item NIELSEN, DANIEL - Orise Fellow
item MAKI, JOEL - Orise Fellow
item Looft, Torey
item RICKER, NICOLE - Orise Fellow

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/2019
Publication Date: 1/2/2020
Citation: Nielsen, D.W., Maki, J.J., Looft, T.P., Ricker, N., Sylte, M.J. 2020. Complete genome sequence of Campylobacter jejuni strain NADC 20827 isolated from commercial turkeys. Microbiology Resource Announcements. 9(1):e01403-19. https://doi.org/10.1128/MRA.01403-19.
DOI: https://doi.org/10.1128/MRA.01403-19

Interpretive Summary: Campylobacter jejuni remains the main bacterial food borne pathogen in humans. Ingestion of contaminated poultry products is the most common route by which humans are infected. We previously colonized turkeys with different strains of Campylobacter that colonized their intestinal tract. Whole genome sequencing of a turkey-derived strain of Campylobacter may be useful to identify which bacterial genes are important to colonize the turkey intestinal tract. The genome of Campylobacter jejuni strain NADC 20827 was sequenced using next generation sequencing technologies. We discovered that this strain contains two plasmids and its genome is larger than most other strains of Campylobacter jejuni. Our results provide vital information for scientists to better understand genetic factors responsible for Campylobacter colonization in turkeys, which may lead to development of intervention strategies to promote a safe food supply and benefit consumers of retail turkey products.

Technical Abstract: Campylobacter jejuni is the main cause of bacterial food-borne disease in humans who are mostly exposed by consumption of contaminated poultry products. C. jejuni strain NADC 20827 was isolated from feces of turkeys naturally colonized with Campylobacter. We present the complete annotated genome and plasmid sequences of strain NADC 20827.