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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Endemic Poultry Viral Diseases Research » Research » Publications at this Location » Publication #370461
Research Project: Enhancing Genetic Resistance to Marek’s Disease in Poultry

Location: Endemic Poultry Viral Diseases Research

Title: TRANSCRIPTOMIC PROFILES AND DIFFERENTIAL GENE EXPRESSION OF SPLENIC CD4+ T CELLS IN RESPONSE TO VACCINATION AGAINST MAREK’S DISEASE

Author
item Zhang, Huanmin
item DONG, KUNZHE - Augusta University
item XIE, QINGMEI - South China Agricultural Univerisity
item CHANG, SHUANG - Shandong Agricultural University
item ZHANG, LEI - Chinese Academy Of Agricultural Sciences

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/26/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Marek’s disease (MD) is a contagious viral neoplastic disease of chicken, which is characterized with T-cell lymphoma. Vaccination has been employed as the primary control of MD since the 1970s. MD vaccine efficacy, however, varies attributable to factors including chicken genetics. To elucidate genomic mechanism underlying vaccine efficacy, this study was designed to probe the transcriptomic differences between two highly inbred lines of White Leghorns that exhibited striking difference in MD incidence in response to HVT vaccination followed by MDV challenge. The two inbred lines, line 63 and line 72, share a common HMC B*2-haplotype. Spleen tissues were sampled from birds 10-days post inoculation of 2,000 PFU HVT or CVI988/Rispens and used in isolation of CD4+ T cells for total RNAs. The total RNA samples were subjected to deep sequencing on an Illumina NextSeq sequencer. All pass-filter reads were subjected to mapping, quantification and differential expression tests followed by functional profiling. A total of 12,024 genes/transcripts was identified. CVI988/Rispens and HVT induced 39 and 57 differentially expressed genes in line 63 birds, 1,337 and 80 genes in line 72 birds, respectively. Differentially expressed genes in response to CVI988/Rispens were enriched in 20 and 695 biological function terms, and in HVT, in 83 and 67 functional terms for lines 63 and 72, respectively, which included biological term of defense response; reactome pathways of innate immune system, immune system, and advanced glycosylation endproduct receptor signaling; and transcriptional factor PU.1, which functions as a major transcriptional activator of tumor suppressor gene.