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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #370541
Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Assessment of genetic diversity by using EST-SSR markers in Lupinus

Author
item ZHANG, HY - Chinese Academy Of Agricultural Sciences
item YANG, T - Chinese Academy Of Agricultural Sciences
item LIU, R - Chinese Academy Of Agricultural Sciences
item JIN, F - National Agro-Tech Extension & Service Centre
item ZHANG, LK - National Agro-Tech Extension & Service Centre
item YU, HT - Yunnan Academy Of Agriculture Sciences
item Hu, Jinguo
item YANG, F - Yunnan Academy Of Agriculture Sciences
item HE, YH - Yunnan Academy Of Agriculture Sciences
item ZONG, X - Chinese Academy Of Agricultural Sciences

Submitted to: Acta Anatomica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/26/2019
Publication Date: 3/12/2020
Citation: Zhang, H., Yang, T., Liu, R., Jin, F., Zhang, L., Yu, H., Hu, J., Yang, F., He, Y., Zong, X. 2020. Assessment of genetic diversity by using EST-SSR markers in Lupinus. Acta Anatomica. 46(3):330-340.

Interpretive Summary: This paper reports the genetic diversity of a subset of 133 Lupinus germplasm accessions belonging to 22 species assessed with expressed sequence tag (EST) -derived simple sequence repeat (SSR) markers. Ninety-five primer pairs amplified 1,318 polymorphic alleles from the subset and the polymorphism information content (PIC) of each allele averaged at 0.75, suggesting a high level of genetic diversity. Subsequent analyses revealed the evolutionary relationship among the 22 species, seventy-seven accessions of seven Lupinus species from the Old World were divided into 4 groups and there was no overlap of accession from different species contained in each identified group. This result is consistent with previous studies.

Technical Abstract: In order to explore the evolutionary relationship of Lupinus preliminarily as well as to excavate and utilize lupin resources from the Old World effectively, the genetic diversity among the species under Lupinus genus was analyzed. Ninety-five polymorphic pairs of EST-SSR markers developed based on the transcriptome of narrow-leaved lupin (Lupinus angustifolius L.) were used to scan 133 lupin accessions from 22 species. A total of 1318 alleles were detected with 13.87 alleles per locus on average, ranging from 3 to 37 alleles; the polymorphism information content (PIC) ranged from 0.39 to 0.91 with the mean value of 0.75; the genetic diversity ranged from 0.41 to 0.92 with the mean value of 0.78. This study showed evolutionary relations among the 22 species under Lupinus genus from the Old World and the New World based on Neighbor-Joining (NJ) method, which is consistent with previous studies. Moreover, seventy-seven lupin accessions of seven Lupinus species from the Old World were divided into 4 groups; there was no overlap of accession from different species contained in each identified group, detected by all the three analysis methods like population structure, cluster analysis based on UPGMA and principal component analysis (PCA).