The development of a diagnostic assay using oral fluids for the detection of Senecavirus A

Authors: ZELENKA, ASHLEY - Kansas State University; Buckley, Alexandra; HUANG, YAN-JANG - Kansas State University; HETTENBACH, SUSAN - Kansas State University; STACK, JAMES - Kansas State University; HIGGS, STEPHEN - Kansas State University; VANLANDINGHAM, DANA - Kansas State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/25/2019
Publication Date: 11/2/2019
Citation: Zelenka, A.M., Buckley, A.C., Huang, Y.S., Hettenbach, S.M., Stack, J., Higgs, S., Vanlandingham, D.L. 2019. The development of a diagnostic assay using oral fluids for the detection of Senecavirus A [abstract]. Great Plains Infectious Disease meeting. Paper No. 1.

Interpretive Summary:

Technical Abstract: Senecavirus A (SVA), formerly Seneca Valley virus (SVV), is the only virus identified in the genus Senecavirus found within the Picornaviridae family. SVA is a vesicular virus affecting swine and is clinically indistinguishable from other vesicular diseases such as Foot and Mouth Disease virus, Vesicular Stomatitis virus, and Swine Vesicular Disease. Since 2015, outbreaks have been reported in Brazil, Thailand, Columbia, China, and the United States of America. Due to its clinical resemblance to other economically devastating Transboundary Animal Diseases, a rapid diagnostic assay that distinguishes SVA infection from other vesicular diseases will facilitate the prevention and control of this virus. The objective of this study is to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that targets the VP2 and 3D-3’UTR region of SVV-001. Oligonucleotide primers were designed using alignment software, comparing highly conserved regions among available isolates. Specificity and sensitivity of different primer sets to complementary cDNA of viral genomes will be discussed.