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Research Project: Monitoring and Molecular Characterization of Antimicrobial Resistance in Foodborne Bacteria

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Title: Draft genome sequences of two potentially novel Bacillus isolates from backyard and commercial chicken gastrointestinal tracts

Author
item KESHRI, JITENDRA - Western University Of Health Sciences
item RAMIREZ, ROCIO - Western University Of Health Sciences
item Berrang, Mark
item OAKLEY, BRIAN - Western University Of Health Sciences

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/2/2020
Publication Date: 5/28/2020
Citation: Keshri, J., Ramirez, R., Berrang, M.E., Oakley, B. 2020. Draft genome sequences of two novel Bacillus isolates from backyard and commercial chicken GIT. Microbiology Resource Announcements. https://doi.org/10.1128/MRA.00492-20.
DOI: https://doi.org/10.1128/MRA.00492-20

Interpretive Summary: Bacillus is a genus of biochemically active bacteria that are found in many environments including the intestines of birds. Determining the genome DNA sequence of these organisms will help to understand how they contribute to the intestinal microbial community. Two novel isolates of Bacillus were isolated from avian gut material, one from goose feces and another from chicken intestine. Their genomic DNA was purified, sequence determined and after rigorous quality control the data was used for de novo genome assembly. Upon comparison of the genome sequence of the current isolates to other published sequences, the isolates were not found to be members of any previously reported species cluster. Thousands of functional protein coding genes were identified. This data will be useful for further studies to determine the function of these isolates in the intestinal microflora of birds. Such insight may shed light on interplay between intestinal bacteria and help further our efforts to understand how pathogens may establish themselves in the intestines or be excluded.

Technical Abstract: Here, we present the draft genome sequences of two Bacillus strains HF117_J1_D and USDA818B3_A isolated in Pomona, California, USA from the GI tracts of backyard and commercial broiler chickens, respectively. The draft genomes of both strains appear to represent novel species.