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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #371702
Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Development of a novel isothermal molecular assay with an internal control to detect tomato brown rugose fruit virus

Author
item LI,, RUGANG - Agdia
item DAVENPORT, BRYANT - Agdia
item ZHANG, SHULU - Agdia
item SCHUETZ, KEITH - Agdia
item Ling, Kai-Shu

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2020
Publication Date: 7/1/2020
Citation: Li,, R., Davenport, B., Zhang, S., Schuetz, K., Ling, K. 2020. Development of a novel isothermal molecular assay with an internal control to detect tomato brown rugose fruit virus. Phytopathology 110:7S1.14
DOI: https://doi.org/10.1094/PHYTO-110-7-S1.1

Interpretive Summary:

Technical Abstract: Tomato brown rugose fruit virus (ToBRFV) is a newly reported Tobamovirus in Jordan in 2015. The subsequent reports from Asian, American, and European countries indicated that ToBRFV has already been spread in the world. The serious damage to tomato and pepper plants including fruits, the resistance-breaking ability, and the seed-spread nature of ToBRFV caused a huge concern within the government organizations, the seed production industries, and tomato growers worldwide. In order to facilitate a reliable detection of ToBRFV in tomato and pepper plants and thus effectively managing ToBRFV disease, Agdia is developing ToBRFV-specific ELISA assay and ImmunoStrip as well as ToBRFV-specific isothermal AmplifyRP assay. The goal for AmplifyRP assay is to target on two virus-specific genes and one host gene as internal control that can detect ToBRFV in crude extract of leaves, fruits, and seeds. The primers and probes were designed from four conserved viral genome regions. The screening experiments discovered two primer/probe combinations located in two separate genomic regions that exhibited specific detection for ToBRFV, but no cross-reactions to other ten Tobamoviruses such as Tobacco mosaic virus, Tomato mosaic virus, and Pepper mild mottle virus. The optimization of primers and probes combinations to achieve a sensitive and reliable detection for ToBRFV will be reported.