Location: Poisonous Plant Research
Title: Analysis of rumen contents and ocular fluid for toxic alkaloids from goats and cows dosed larkspur (Delphinium berbeyi), lupine (Lupinus leucophyllus), and death camas (Zigadenus paniculatus)Author
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Submitted to: Toxicon
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 1/10/2020 Publication Date: 1/11/2020 Citation: Lee, S.T., Welch, K.D., Stonecipher, C.A., Cook, D., Gardner, D.R., Pfister, J.A. 2020. Analysis of rumen contents and ocular fluid for toxic alkaloids from goats and cows dosed larkspur (Delphinium berbeyi), lupine (Lupinus leucophyllus), and death camas (Zigadenus paniculatus). Toxicon. 176: 21-29. https://doi.org/10.1016/j.toxicon.2020.01.003. DOI: https://doi.org/10.1016/j.toxicon.2020.01.003 Interpretive Summary: Larkspurs, lupines, and death camas can be acutely toxic to livestock and are serious poisonous plant problems in western North America. The toxicity of these plants depends on the composition and concentrations of the toxic alkaloids in the plants. In this study, goats and cows were dosed sub-lethal doses of larkspur, lupine, and death camas. Rumen contents and ocular fluid samples were collected, and simple extraction, sample preparation, and analytical methods were developed for the detection of toxic alkaloids in the rumen contents and ocular fluid samples. Toxic alkaloids were detected in the rumen contents and ocular fluid samples from the goats and cows dosed larkspur, lupine, and death camas. In addition, results from a case report where rumen contents were analyzed from a steer that was suspected to have died due to larkspur are reported. This demonstrates the utility of the methods described for the diagnosis of acute plant poisonings. Technical Abstract: Larkspurs, lupines, and death camas can be acutely toxic to livestock and are serious poisonous plant problems in western North America. The toxicity of these plants depends on the composition and concentrations of the toxic alkaloids in the plants. In this study, goats and cows were dosed sub-lethal doses of larkspur, lupine, and death camas. Rumen contents and ocular fluid samples were collected, and simple extraction, sample preparation, and analytical methods were developed for the detection of toxic alkaloids in the rumen contents and ocular fluid samples. Toxic alkaloids were detected in the rumen contents and ocular fluid samples from the goats and cows dosed larkspur, lupine, and death camas. In addition, results from a case report where rumen contents were analyzed from a steer that was suspected to have died due to larkspur are reported. This demonstrates the utility of the methods described for the diagnosis of acute plant poisonings. |
