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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #373640
Research Project: Development of Economically Important Row Crops that Improve the Resilience of U.S. Agricultural Production to Present and Future Production Challenges

Location: Plant Stress and Germplasm Development Research

Title: Detection of potentially pathogenic efector genes in Fusarium oxysporum f. sp. vasinfectum race 4 isolates from California

Author
item URNER, MICHAEL - California State University
item HUTMACHER, ROBERT - University Of California
item Ulloa, Mauricio
item NICHOLS, ROBERT - Cotton, Inc
item ELLIS, MARGARET - California State University

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/14/2020
Publication Date: 8/3/2020
Citation: Urner, M., Hutmacher, R.B., Ulloa, M., Nichols, R., Ellis, M.L. 2020. Detection of potentially pathogenic efector genes in Fusarium oxysporum f. sp. vasinfectum race 4 isolates from California. APS Annual Meeting - Plant Health Conference. Denver, CO, USA. 10-14, August 2020. 17406.

Interpretive Summary:

Technical Abstract: Fusarium oxysporum f. sp. vasinfectum (FOV) race 4 is an important soilborne pathogen responsible for Fusarium wilt of cotton. So far, FOV race 4 is geographically limited, first identified in California in 2001, and later confirmed in Texas in 2017 and New Mexico in 2019. The objective of this research was to screen FOV race 4 isolates collected from cotton across the San Joaquin Valley of California for possible fungal effectors that might be associated with disease development in cotton. These effectors included the Six genes that are cysteine rich proteins secreted in the xylem (SIX) of infected plants. A total of 74 FOV race 4 isolates were screened for the following known fungal effector genes Six1, Six2, Six3, Six4, Six6, Pda1, Pep1, and Prt1 using specific PCR specific primer-pair primers. The effector genes Six1, Six2, Six4, and Six6 were not present in the 74 FOV race 4 isolates tested, while Pep1 was dispersed among isolates. The fungal effector genes Six3, Prt1, and Pda1 were present in the majority of the 74 FOV race 4 isolates. Future work should examine the role of these three fungal effectors and their potential role in pathogenicity towards cotton. A better understanding of the fungal effectors involved in pathogenicity will also aid in the development of more durable and resistant cotton germplasm that can be used to manage this important wilt disease.