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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #374158
Research Project: Improving Control of Stripe Rusts of Wheat and Barley through Characterization of Pathogen Populations and Enhancement of Host Resistance

Location: Wheat Health, Genetics, and Quality Research

Title: Genome-wide mapping of quantitative trait loci conferring all-stage and high-temperature adult-plant resistance to stripe rust in spring wheat landrace PI 181410

Author
item LIU, YAN - Washington State University
item QIE, YANMIN - Washington State University
item LI, XIN - Washington State University
item WANG, MEINAN - Washington State University
item Chen, Xianming

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/9/2020
Publication Date: 1/12/2020
Citation: Liu, Y., Qie, Y., Li, X., Wang, M., Chen, X. 2020. Genome-wide mapping of quantitative trait loci conferring all-stage and high-temperature adult-plant resistance to stripe rust in spring wheat landrace PI 181410. International Journal of Molecular Sciences. 21(2):478. https://doi.org/10.3390/ijms21020478.
DOI: https://doi.org/10.3390/ijms21020478

Interpretive Summary: Stripe rust is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and non-race specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with susceptible wheat variety Avocet S (AvS), from which a recombinant inbred line (RIL) population was developed. The RIL population was phenotyped for stripe rust response in multiple field environments under natural infection of the stripe rust pathogen, and was phenotyped for stripe rust response in seedlings at low-temperature and in adult-plant stage with selected races of the pathogen in the greenhouse. The population was genotyped using the 90K wheat SNP chip. Three QTL from PI 181410 for all stage resistance were mapped on chromosome arms 4AS, 4BL, and 5BL, and four QTL were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three Kompetitive Allele Specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.

Technical Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and non-race specific hightemperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5 – F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low-temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1 from PI 181410 for all stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three Kompetitive Allele Specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.