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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Sustainable Perennial Crops Laboratory » Research » Publications at this Location » Publication #374402
Research Project: Develop Pest Management Technologies and Strategies to Control the Coffee Berry Borer

Location: Sustainable Perennial Crops Laboratory

Title: Draft genome of pseudomonas sp. Strain ces, containing the entire alkylxanthine gene cluster for caffeine breakdown

Author
item SUMMERS, RYAN - University Of Alabama
item Shao, Jonathan
item MOCK, MERESITH - University Of Alabama
item YU, CHI-LI - University Of Alabama
item Vega, Fernando

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/22/2020
Publication Date: 7/9/2020
Citation: Summers, R., Shao, J.Y., Mock, M., Yu, C., Vega, F.E. 2020. Draft genome of pseudomonas sp. Strain ces, containing the entire alkylxanthine gene cluster for caffeine breakdown. Microbiology Resource Announcements. https://doi.org/10.1128/MRA.
DOI: https://doi.org/10.1128/MRA

Interpretive Summary: Several bacteria have been shown to be able to breakdown caffeine. We sequenced the genome of a Pseudomonas strain capable of breaking down caffeine and identified several important components related to the genome. Among these, we found five different genes involved in caffeine breakdown. Availability of the Pseudomonas strain genome could be helpful in elucidating the molecular evolution of Pseudomonas species as well as when sampling other bacteria for caffeine breakdown genes. This information will be of use to biochemists, entomologists, molecular biologists, and microbiologists.

Technical Abstract: Pseudomonas strain CES was isolated from Caffeine Enriched Soil and found to possess the N-demethylation pathway for caffeine breakdown. We report the nucleotide sequence of the draft genome with 5,827,822 base pairs, 62.6% G+C, and 5,427 protein coding regions.