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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #374464
Research Project: Improvement of Biotic Stress Resistance in Durum and Hard Red Spring Wheat Using Genetics and Genomics

Location: Cereal Crops Research

Title: Genetic mapping of spot form net blotch resistance using a bi-parental population

Author
item ALHASHEL, ABDULLAH - North Dakota State University
item PODEL, ROSHAN - North Dakota State University
item CLARE, SHAUN - Washington State University
item Friesen, Timothy
item Yang, Shengming
item BRUEGGEMAN, ROBERT - Washington State University

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/6/2020
Publication Date: 8/10/2020
Citation: Alhashel, A., Podel, R., Clare, S., Friesen, T.L., Yang, S., Brueggeman, R. 2020. Genetic mapping of spot form net blotch resistance using a bi-parental population [abstract]. Plant Health 2020. 16075.

Interpretive Summary:

Technical Abstract: Spot form net blotch (SFNB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. maculata (Ptm) is a foliar pathogen of barley that causes significant yield losses in major barley growing regions worldwide. Understanding the host-parasite interactions between pathogen virulence/avirulence genes and the corresponding susceptibility/resistance genes in the host is important for the deployment of genetic resistance against SFNB. A recombinant inbred mapping population derived from CIho5791 (S) × Tifang (R) was developed to characterize genetic resistance/susceptibility to the Ptm isolate 13IM8.3, which was collected from Idaho (ID). This isolate was selected based on its distinct virulence profile from others collected from three states: Idaho, Montana (MT), and North Dakota (ND). Quantitative trait locus (QTL) analysis of an F6 recombinant inbred line (RILs) population identified one resistance or susceptibility locus each on chromosome 4H, 6H and 7H. However, phenotyping of F2 individuals showed a bi-modal distribution with ~50% of the progenies having higher levels of susceptibility than the susceptible parent CIho5791. PCR-GBS analysis of the F2 individuals revealed one heterozygous locus on the 5H chromosome correlated with high levels of susceptibility, indicating the susceptibility to Ptm isolate 13IM8.3 may be caused by an allelic interaction at this locus. Loci identified in this study will help facilitate the deployment of resistance in barley to SFNB.