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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #374554
Research Project: Mitigating High Consequence Domestic, Exotic, and Emerging Diseases of Fruits, Vegetables, and Ornamentals

Location: Subtropical Plant Pathology Research

Title: Genome characterization of Brugmansia latent virus, a novel tobamovirus

Author
item SCOTT-BROWN, ALISON - Royal Botanical Gardens
item D'ELIA, TOM - Indian River State College
item DEVEY, DION - Royal Botanical Gardens
item FUNDERBURK, JOSEPH - University Of Florida
item Adkins, Scott

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/30/2020
Publication Date: 7/22/2020
Citation: Scott-Brown, A.S., D'Elia, T., Devey, D.S., Funderburk, J.E., Adkins, S.T. 2020. Genome characterization of Brugmansia latent virus, a novel tobamovirus. Archives of Virology. https://doi.org/10.1007/s00705-020-04718-z.
DOI: https://doi.org/10.1007/s00705-020-04718-z

Interpretive Summary: The full genome of Brugmansia latent virus (BrLV) isolated from Brugmansia in the United Kingdom is presented. Related to but distinct from several other viruses that also infect this ornamental crop plant, a complete genome sequence for BrLV has been lacking until this report. The BrLV full genome sequence shows this virus to be a new species and will enable further ecological and epidemiological studies, and also the development of additional diagnostic tests.

Technical Abstract: A novel tobamovirus, Brugmansia latent virus (BrLV), was discovered during a study of Brugmansia (Brugmansia spp.) at the Royal Botanic Gardens, Kew, Surrey, UK. Here we report the complete genome sequence of BrLV, which is 6,397 nucleotides long and contains the four open reading frames (RNA-dependent RNA polymerase, methyltransferase/helicase, movement, and coat proteins) typical of tobamoviruses. The complete genome sequence of BrLV shares 69.7% nucleotide sequence identity with Brugmansia mild mottle virus (BrMMV), and 66.7 to 68.7% identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the complete genome nucleotide sequence and the deduced amino acid sequences of the four tobamovirus proteins place BrLV in a subcluster with BrMMV within the Solanaceae-infecting tobamovirus subgroup as a new species.