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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #374742
Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Complete genome sequence of a tomato brown rugose fruit virus isolated in the United States

Author
item Chanda, Bidisha
item RIVERA, YAZMIN - Animal And Plant Health Inspection Service (APHIS)
item NUNZIATA, SCHYLER - Animal And Plant Health Inspection Service (APHIS)
item GALVEZ, MARCO - Animal And Plant Health Inspection Service (APHIS)
item Gilliard, Andrea
item Ling, Kai-Shu

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/25/2020
Publication Date: 7/16/2020
Citation: Chanda, B., Rivera, Y., Nunziata, S.O., Galvez, M.E., Gilliard, A.C., Ling, K. 2020. Complete genome sequence of a tomato brown rugose fruit virus isolated in the United States. Microbiology Resource Announcements. 9(29):e00630-20. https://doi.org/10.1128/mra.00630-20.
DOI: https://doi.org/10.1128/mra.00630-20

Interpretive Summary: Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops, with the United States as the third largest producer in the world. Tomato is especially susceptible to many viral diseases, which are difficult to manage. In the last few years, a new species of tobamovirus, tomato brown rugose fruit virus (ToBRFV), originally identified in the Middle East since 2014, has been identified to cause outbreaks in greenhouse tomatoes around the world, including the U.S. In this study, ARS scientists at the U.S. Vegetable Laboratory, in collaboration with USDA-APHIS researchers sequenced the genome of the U.S. isolate of ToBRFV, evaluated its phylogenetic relationship with other tobamoviruses. Such fundamental knowledge on the molecular properties of ToBRFV are necessary to manage this emerging viral disease.

Technical Abstract: Complete genome sequence of a U.S. isolate of tomato brown rugose fruit virus (ToBRFV, CA18-01) was obtained through Illumina and MinION sequencing. The ToBRFV U.S. isolate shared a high sequence identity (>99%) with all known ToBRFV isolates. Phylogenetic analysis revealed a tight cluster for ToBRFV isolates from around the world suggesting a short evolutionary history.