The Hessian fly recessive resistance gene H4 mapped to chromosome 1A of the wheat cultivar ‘Java’ using genotyping-by-sequencing

Authors: NIU, FUAN - Kansas State University; XU, YUNFENG - Kansas State University; Liu, Xuming; ZHAO, LANFEI - Kansas State University; Bernardo, Amy; YAOGUANG, LI - Kansas State University; GUIXIA, LIU - Kansas State University; Chen, Ming-Shun; CAO, LIMING - Shanghai Academy Of Agricultural Sciences; HU, ZHENBIN - Kansas State University; Xu, Xiangyang; Bai, Guihua

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/20/2020
Publication Date: 7/2/2020
Citation: Niu, F., Xu, Y., Liu, X., Zhao, L., Bernardo, A.E., Yaoguang, L., Guixia, L., Chen, M., Cao, L., Hu, Z., Xu, X., Bai, G. 2020. The Hessian fly recessive resistance gene H4 mapped to chromosome 1A of the wheat cultivar ‘Java’ using genotyping-by-sequencing. Journal of Theoretical and Applied Genetics. https://doi.org/10.1007/s00122-020-03642-9.
DOI: https://doi.org/10.1007/s00122-020-03642-9

Interpretive Summary: Hessian fly (HF) is an important destructive insect in wheat worldwide. Genetic resistance is one of the best control methods for HF. To determine the chromosome location of a HF resistance gene (h4) in wheat cultivar ‘Java’, we analyzed two wheat populations developed from crosses of ‘Bobwhite’ × Java and ‘Overley’ × Java and confirmed that h4 is a recessive gene. We mapped h4 to a short region on the short arm of chromosome 1A and the gene explained 60.4 to 70.5% of the variation for HF resistance. We developed two breeder-friendly KASP markers for pyramiding h4 with other HF resistance genes in breeding to improve HF resistance in wheat.

Technical Abstract: Hessian fly (HF), Mayetiola destructor, is one of the destructive insect pests in wheat worldwide. The wheat cultivar ‘Java’ was reported to carry a recessive gene (h4) for HF resistance, however, its chromosome location has not been determined. To map the HF resistance gene in Java, two populations of recombinant inbred lines (RILs) were developed from ‘Bobwhite’ × Java and ‘Overley’ × Java, respectively, and were phenotyped for responses to infestation of HF Great Plains biotype (GP). Analysis of phenotypic data from the F1 and the RIL populations confirmed that one recessive gene conditioned HF resistance in Java. Two linkage maps were constructed using single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing (GBS). The h4 gene was mapped to the distal end of the short arm of chromosome 1A, which explained 60.4 to 70.5% of the phenotypic variation for HF resistance in the two populations. The GBS-SNPs in the h4 candidate interval were converted into Kompetitive Allele Specific Polymerase Chain Reaction (KASP) markers to eliminate the missing datapoints in GBS-SNPs. Using the revised maps with KASP markers, h4 was further located to a 642 kb interval (6,635,984 – 7,277,935 bp). The two flanking KASP markers, KASP3299 and KASP1871, as well as four other closely-linked KASP markers, are useful f or pyramiding h4 with other HF resistance genes in breeding.