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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #374991
Research Project: Genetic Mechanisms and Improvement of Insect Resistance in Wheat, Barley, and Sorghum

Location: Peanut and Small Grains Research Unit

Title: Development of KASP markers for wheat greenbug resistance gene Gb5

Author
item Xu, Xiangyang
item Li, Genqiao
item Bai, Guihua
item Bernardo, Amy
item CARVER, BRETT - Oklahoma State University
item St Amand, Paul
item Armstrong, John

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/8/2020
Publication Date: 9/8/2020
Citation: Xu, X., Li, G., Bai, G., Bernardo, A., Carver, B.F., St Amand, P., Armstrong, S. 2020. Development of KASP markers for wheat greenbug resistance gene Gb5. Crop Science. 61(1):490–499. https://doi.org/10.1002/csc2.20339.
DOI: https://doi.org/10.1002/csc2.20339

Interpretive Summary: Greenbug [Schizaphis graminum (Rondani)] is a destructive pest of wheat worldwide. Incorporation of wheat greenbug resistance genes in wheat breeding pipelines is critical to improve wheat production. The greenbug resistance gene Gb5 resides in a wheat-T. speltoildes chromosome segment and confers resistance to several economically important greenbug biotypes. This study characterized the T. speltoides chromosome segment in PI 603919 and developed KASP markers to facilitate its introgression in wheat breeding. A set of 21 SSR markers specific to wheat 7AL chromosome were developed to determine the length and location of the T. speltoides chromosome segment, and the T. speltoides segment in PI 603919 was estimated to be about 79.5-87.8 Mb in the Chinese Spring reference sequence IWGSC RefSeq v.1.0. Three KASP markers were developed to tag the wheat-T. speltoildes chromosome segment, and validation tests indicated that these KASP markers can be used to select Gb5 in wheat breeding.

Technical Abstract: Greenbug [Schizaphis graminum (Rondani)] is a destructive pest of wheat worldwide. Incorporation of wheat greenbug resistance genes in wheat breeding pipelines is critical to improve wheat production. The greenbug resistance gene Gb5, originally identified in Triticum speltoides chromosome 7S and transferred to wheat chromosome 7A, confers resistance to several economically important greenbug biotypes. The T. speltoides chromosome segment carrying Gb5 was previously shortened by means of homeologous recombination induced by the ph1b gene, which minimized linkage drag and made Gb5 a useful gene for improvement of greenbug resistance in wheat. This study characterized the shortened T. speltoides chromosome segment in PI 603919 and developed KASP markers to facilitate its introgression in wheat breeding. A set of 21 SSR markers specific to wheat 7AL chromosome were developed to determine the length and location of the T. speltoides chromosome segment, and the T. speltoides segment in PI 603919 was estimated to be about 79.5-87.8 Mb in the Chinese Spring reference sequence IWGSC RefSeq v.1.0. A recombinant inbred line (RIL) population derived from a cross between CI 17884, which carries the original wheat-T. speltoides 7A-7S translocation chromosome segment, and Bainong 418 was genotyped using the genotyping-by-sequencing (GBS) approach and evaluated for responses to greenbug biotype E. Three GBS-SNPs on the shortened translocation segment were converted to KASP markers. These KASP markers were validated and can be used to select Gb5 in wheat breeding.