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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #375119
Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Detection and characterization of a second carlavirus in Rosa sp.

Author
item DIAZ-LARA, ALFREDO - University Of California, Davis
item Mollov, Dimitre
item GOLINO, DEBORAH - University Of California, Davis
item AL RWAHNIH, MAHER - University Of California, Davis

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/18/2020
Publication Date: 11/11/2020
Citation: Diaz-Lara, A., Mollov, D.S., Golino, D., Al Rwahnih, M. 2020. Detection and characterization of a second carlavirus in Rosa sp. Archives of Virology. https://doi.org/10.1007/s00705-020-04864-4.
DOI: https://doi.org/10.1007/s00705-020-04864-4

Interpretive Summary: Roses (Rosa spp.), one of the most popular ornamentals worldwide, are used as both cut flowers and landscape plants. Many viral diseases affect rose production. It is important to detect and eliminate virus infected roses prior to material being propagated and distributed. We report a new virus identified in rose and provisionally named Rose virus B (RVB). The RVB genomic organization and sequence was determined. This study provides information for the proper classification of RVB. The genome sequence facilitates developing reliable detection methods that can be used to screen rose genetic material. The entire rose germplasm collection at Foundation Plant Services at the University of California-Davis was tested for RVB and seven rose accessions were determined RVB positive. This information is useful for developing better viral control measures in the rose trade and production industries.

Technical Abstract: A new virus resembling species in the genus Carlavirus was identified in an ‘Out of Yesteryear’ rose (Rosa sp.) by high throughput sequencing. The virus was discovered during the screening of a rose virus collection belonging to Foundation Plant Services (University of California-Davis). The full genome of the virus is 8825 nt long, excluding a poly (A) tail, and includes six predicted genes: replicase, triple gene block (TGB1-3), coat protein (CP) and nucleic acid binding protein. The closest relative of the putative virus is rose virus A (RVA; genus Carlavirus) with 75% and 78% aa identity in the replicase and CP, respectively. The relationship with RVA and other carlaviruses was supported by phylogenetic analyses using replicase and CP sequences. Based on genome organization, sequence identity and phylogenetic analysis, the virus found in the ‘Out of Yesteryear’ plant represents a new member of the genus Carlavirus and is provisionally named “rose virus B” (RVB). Further testing by reverse transcription PCR confirmed the presence of RVB in the original source and seven additional rose selections located at the same collection.