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ARS Home » Plains Area » Grand Forks, North Dakota » Grand Forks Human Nutrition Research Center » Dietary Prevention of Obesity-related Disease Research » Research » Publications at this Location » Publication #375625
Research Project: Modification of Diurnal Patterns to Promote Health in Models for Human Metabolic Dysfunction

Location: Dietary Prevention of Obesity-related Disease Research

Title: Osteoprotective effect of green tea polyphenols and annatto-extracted tocotrienol in obese mice via enhancing the microbiome vitamin K2 biosynthetic pathways

Author
item ELMASSRY, MOAMEN - Texas Tech University
item CHUNG, EUNHEE - University Of Texas At San Antonio
item Cao, Jay
item HAMOOD, ABDUL - Texas Tech University
item SHEN, CHWAN-LI - Texas Tech University

Submitted to: Journal of Nutritional Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/18/2020
Publication Date: 9/11/2020
Citation: Elmassry, M.M., Chung, E., Cao, J.J., Hamood, A.N., Shen, C. 2020. Osteoprotective effect of green tea polyphenols and annatto-extracted tocotrienol in obese mice via enhancing the microbiome vitamin K2 biosynthetic pathways. Journal of Nutritional Biochemistry. 86. Article 108492. https://doi.org/10.1016/j.jnutbio.2020.108492.
DOI: https://doi.org/10.1016/j.jnutbio.2020.108492

Interpretive Summary: The gut microbiome plays an important role in nutrient absorption, immune system, and bone health. We previously showed that green tea polyphenols (GTP), a compound in tea, and annatto-extracted tocotrienols (AT), a form of vitamin E, are beneficial to bone. In this study, we investigated the effects of GTP and AT on bone structure and gut microbial composition in obese male mice. We found that AT and GTP supplementation increased bone mass. GTP supplementation also increased serum concentration of bone formation marker, procollagen type 1 N-terminal propeptide. ATP and AT supplementation increased the population of gut microbe, Akkermansia muciniphila. Studies show that Akkermansia muciniphila improves glucose homeostasis and reduces inflammation in old age and obesity. Our results suggest that supplementation of GTP and AT is beneficial to bone health in obese mice likely by favorably modifying gut microbiome composition and function.

Technical Abstract: This study investigated the effects of green tea polyphenols (GTP) and annatto-extracted tocotrienols (AT) on bone properties and gut microbiome in obese mice. Male, C57BL/6J mice were fed a high-fat diet and assigned to a 2 (no AT vs. 400 mg AT/kg diet) × 2 (no GTP vs. 0.5%, w/v GTP in drinking water) factorial design, namely control, G, T, and G+T groups, for 14 weeks. The 4th lumbar vertebra (LV-4) and femur were harvested for bone microstructural analysis using micro CT instrument. Microbiome analysis using 16S rRNA gene sequencing of cecal feces was performed. AT supplementation increased bone volume at the distal femur. GTP supplementation increased serum procollagen type 1 N-terminal propeptide concentration, bone volume at distal femur and LV-4, and trabecular number at distal femur; whereas GTP decreased trabecular separation at distal femur. Interactions between GTP and AT supplementation were found in serum C-terminal telopeptide of type I collagen level (control>G=T=G+T) as well as the cortical bone area (control