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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #375865
Research Project: Maximizing the Impact of Potato Genebank Resources: Development and Evaluation of a Wild Species Genotype Diversity Panel

Location: Vegetable Crops Research

Title: Mapping of QTLs controlling epicotyl length in adzuki bean (Vigna angularis)

Author
item MORI, MASAHIKO - Obihiro University Of Agriculture And Veterinary Medicine
item MAKI, KENTO - Obihiro University Of Agriculture And Veterinary Medicine
item KAWAHATA, TSUBASA - Obihiro University Of Agriculture And Veterinary Medicine
item KAWAHARA, DAIGO - Obihiro University Of Agriculture And Veterinary Medicine
item KATO, YUTA - Obihiro University Of Agriculture And Veterinary Medicine
item YOSHIDA, TORU - Obihiro University Of Agriculture And Veterinary Medicine
item NAGASAWA, HIDETAKA - Hokkaido Research Organization
item SATOH, HITOSHI - Hokkaido Research Organization
item NAGANO, ATUSHI - Ryukoku University
item Bethke, Paul
item KATO, KIYOOAKI - Obihiro University Of Agriculture And Veterinary Medicine

Submitted to: Breeding Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/2/2020
Publication Date: 4/7/2021
Citation: Mori, M., Maki, K., Kawahata, T., Kawahara, D., Kato, Y., Yoshida, T., Nagasawa, H., Satoh, H., Nagano, A., Bethke, P.C., Kato, K. 2021. Mapping of QTLs controlling epicotyl length in adzuki bean (Vigna angularis). Breeding Science. 71(2):208-216. https://doi.org/10.1270/jsbbs.20093.
DOI: https://doi.org/10.1270/jsbbs.20093

Interpretive Summary: Mechanical weeding and harvest of adzuki beans would be easier if the stem at the base of seedlings and mature plants was longer. The length of this part of the stem is genetically controlled, but the precise genes involved are not known. In this research, four locations within the adzuki bean DNA that contribute to greater stem length were identified, as were molecular markers linked to those four locations. These findings will assist breeders of adzuki bean to develop varieties that are better suited for mechanical weeding and harvesting. This, in turn, will increase the efficiency of adzuki bean production, benefiting growers and consumers of adzuki beans.

Technical Abstract: The epicotyl length (ECL) of the adzuki bean (Vigna angularis) affects its mechanized weeding at the seedling stage and its harvestings. The present study investigated the genetic factors controlling ECL using a molecular linkage map based on an F2 population derived from a cross between the breeding line, ‘Tokei1121’ (T1121, long epicotyls) and the cultivar ‘Erimo167’ (normal epicotyls). Fifty-two segregating markers, including 27 simple sequence repeats (SSRs) and 25 single-nucleotide polymorphisms (SNPs), were located on eight linkage groups (LGs) at a LOD threshold value of 3.0. Four quantitative trait loci (QTLs) for ECL, with LOD values of 4.0, 3.4, 4.8 and 6.4, were identified on LGs 2, 4, 7 and 10; together, these four QTLs accounted for 49.3% of the phenotypic variance when joined together. The segregation patterns observed in F5 residual heterozygous lines at qECL10 revealed that longer ECL was controlled by a single recessive gene derived from T1121. Using five insertion and deletion markers, this gene was fine mapped to ~255kb at the terminus of LG10. These findings will facilitate the understanding of the mechanisms associated with epicotyl elongation and marker-assisted selection for breeding in the adzuki bean.