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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #376150
Research Project: Umbrella Project for Food Safety

Location: Warmwater Aquaculture Research Unit

Title: Comparing the kinetics of the hydrolysis of by-product from channel catfish (Ictalurus punctatus) fillet processing by eight proteases

Author
item TAN, YUQING - Mississippi State University
item CHANG, SAM - Mississippi State University
item MENG, SHI - Mississippi State University

Submitted to: LWT - Food Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/12/2019
Publication Date: 5/21/2019
Citation: Tan, Y., Chang, S., Meng, S. 2019. Comparing the kinetics of the hydrolysis of by-product from channel catfish (Ictalurus punctatus) fillet processing by eight proteases. LWT - Food Science and Technology. 111:809-820.

Interpretive Summary: Catfish aquaculture is the number one aquaculture in the United States and accounts for more than $400 million at the farm gate. Filleting making added additional value to $1 billion per year. Catfish by-products (heads and frames) account for more than 50% of the total fish weight and have no value. To make value-added products for human consumption, eight different proteases were used for hydrolysis the catfish by-products. Degree of hydrolysis of the hydrolysates and hydrolysis kinetic were studied, and protease cost for reaching certain degree of hydrolysis was predicted. Functional properties of the hydrolysates were evaluated. The results showed useful protein products could be made from the whole ground by products. This research, connecting agriculture/aquaculture and food technology for utilizing agriculture by-products would contribute significantly to agriculture sustainability.

Technical Abstract: Channel catfish farming is the most important warm water aquaculture in the United States and is very important to the rural economy. This study's objective was to investigate the kinetics of the enzymatic hydrolysis of catfish by-product. Channel catfish (Ictalurus punctatus) by-products (heads and frames) were collected from local catfish processing plant. Papain, ficin, bromelain, neutrase, alcalase, protamex, novo-proD and thermolysin were used for hydrolysis. Proteolytic activities of those proteases were examined at different hydrolysis temperature, proteases cost for reaching certain degree of hydrolysis was predicted. Degree of hydrolysis (DH) of the hydrolysates and hydrolysis kinetics were studied. Emulsifying and foaming property and stability of selected hydrolysates were evaluated. Results indicated that thermolysin had the highest activity (820 million Azocoll Units/g and 5.6 million Azocasein Units/g) at 70'°C. The highest DH of the hydrolysates were observed in 120 min with the concentration of 80 AzU/g for all enzymes. Ficin (80 AzU/g) was the most efficient in hydrolyzing the by-product (DH reaching 71%) in 120 'min'at 30'°C. The hydrolysis curves fit the Peleg model well with R2 higher than 0.91. This study provides important engineering information for choosing proteases and processing conditions for optimization of efficiency, yield and economy.