Morphological and physiological responses of Cornus alba to salt and drought stresses under greenhouse conditions

Authors: LIU, QIANG - CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY; SUN, YOUPING - UTAH STATE UNIVERSITY; Altland, James; NIU, GENHUA - TEXAS A&M AGRILIFE

Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/18/2019
Publication Date: 1/10/2020
Citation: Liu, Q., Sun, Y., Altland, J.E., Niu, G. 2020. Morphological and physiological responses of Cornus alba to salt and drought stresses under greenhouse conditions. HortScience. 55(2):224–230. https://doi.org/10.21273/HORTSCI14460-19.
DOI: https://doi.org/10.21273/HORTSCI14460-19

Interpretive Summary: Water scarcity is one of the severe environmental constraints to plant growth and development. Soil salinity is also a global issue and is caused partially by human activities such as irrigation with poor quality water and poor soil drainage, which result in excess soluble salts in the soil. Tatarian dogwood (Cornus alba) is a popular ornamental shrub with white fruits, creamy-white flowers, and red stems in fall through late winter. It is widely used in residential landscape, public parks, and botanical gardens. However, little is known regarding the tolerance of salt and drought stresses of tatarian dogwood. Therefore, the purpose of this study was to characterize the survival, morphological, aesthetic, and physiological responses of tatarian dogwood seedlings to salinity and drought stresses. In Tatarian dogwood seedlings grown in three soilless growing substrates (Metro-Mix 360, 560, and 902) and were irrigated with a nutrient solution at an electrical conductivity (EC) of 1.2 dS·m-1 (control) or saline solution with an EC of 5.0 or 10.0 dS·m-1 once per week for 8 weeks. Seedlings were also irrigated at a substrate volumetric water contents (volume of water/volume of substrate, VWC) of 15%, 20%, 25%, 30%, 35%, 40%, or 45% using a sensor-based automated irrigation system for 60 days. All plants died when irrigated with saline solutions at an EC of 10.0 dS·m-1, and those plants irrigated with saline solutions at an EC of 5.0 dS·m-1 exhibited severe foliar damage. Drought stress decreased plant growth of tatarian dogwood seedlings, but all plants survived at all VWC treatments. On the basis of the results described here, tatarian dogwood can be considered salt sensitive but drought tolerant.

Technical Abstract: Tatarian dogwood (Cornus alba) is an ornamental shrub with white fruits, creamy-white flowers, and red stems in fall through late winter and is widely used in residential landscape, public parks, and botanical gardens. Two greenhouse experiments were conducted to characterize the survival, morphological, aesthetic, and physiological responses of tatarian dogwood seedlings to salinity and drought stresses. In Expt. 1, tatarian dogwood seedlings grown in three soilless growing substrates (Metro-Mix 360, 560, and 902) were irrigated with a nutrient solution at an electrical conductivity (EC) of 1.2 dS·m-1 (control) or saline solution (by adding calculated amount of sodium chloride and calcium chloride) at an EC of 5.0 or 10.0 dS·m-1 once per week for 8 weeks. Results showed that substrate did not influence the growth of tatarian dogwood seedling. All plants irrigated with saline solutions at an EC of 10.0 dS·m-1 died, whereas those irrigated with saline solutions at an EC of 5.0 dS·m-1 exhibited severe foliar salt damage with an average visual score of 1.0 (on a scale of 0 to 5, with 0 = dead and 5 = excellent without foliar salt damage). Compared with the control, saline solutions at an EC of 5.0 dS·m-1 reduced plant height and shoot dry weight (DW) by 50.8% and 55.2%, respectively. Relative chlorophyll content [soil plant analysis development (SPAD) reading], chlorophyll fluorescence (Fv/Fm), and net photosynthesis rate (Pn) also decreased when plants were irrigated with saline solutions at an EC of 5.0 and 10.0 dS·m-1. Leaf sodium (Na+) concentration of tatarian dogwood seedlings irrigated with saline solutions at an EC of 5.0 and 10.0 dS·m-1 increased 11 and 40 times, respectively, compared with the control, whereas chloride (Cl-) concentration increased 25 and 33 times, respectively. In Expt. 2, tatarian dogwood seedlings were irrigated at a substrate volumetric water contents (volume of water/volume of substrate, VWC) of 15%, 20%, 25%, 30%, 35%, 40%, or 45% using a sensor-based automated irrigation system for 60 days. Results showed that drought stress decreased plant growth of tatarian dogwood seedlings with a reduction of 71%, 85%, and 87% in plant height, leaf area, and shoot DW, respectively, when VWC decreased from 45% to 15%, but all plants survived at all VWC treatments. Significant reductions of photosynthesis (Pn), stomatal conductance (gS), transpiration rate (E), and water potential were also found in plants at a VWC of 15%, compared with other VWCs. However, SPAD readings and Fv/Fm of tatarian dogwood seedlings were similar among the VWCs. In conclusion, tatarian dogwood seedlings were sensitive to the salinity levels tested in this study but could survive at all tested substrate volumetric water contents and exhibited resistance to drought conditions.