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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #377229
Research Project: Genetic Improvement of Biotic and Abiotic Stress Tolerance and Nutritional Quality in Hard Winter Wheat

Location: Hard Winter Wheat Genetics Research

Title: Identification of candidate chromosome region of Sbwm1 for Soil-borne wheat mosaic virus resistance in wheat

Author
item LIU, SHUBING - Shangdong Agricultural University
item Bai, Guihua
item LIN, MENG - Kansas State University
item LUO, MINGCHENG - University Of California, Davis
item ZHANG, DADONG - Kansas State University
item JIN, FENG - Kansas State University
item TIAN, BIN - Kansas State University
item TRICK, HAROLD - Kansas State University
item YAN, LIULING - Oklahoma State University

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/20/2020
Publication Date: 5/15/2020
Citation: Liu, S., Bai, G., Lin, M., Luo, M., Zhang, D., Jin, F., Tian, B., Trick, H., Yan, L. 2020. Identification of candidate chromosome region of Sbwm1 for Soil-borne wheat mosaic virus resistance in wheat. Scientific Reports. https://doi.org/10.1038/s41598-020-64993-3.
DOI: https://doi.org/10.1038/s41598-020-64993-3

Interpretive Summary: Soil-borne wheat mosaic virus (SBWMV) can significantly reduce grain yields in winter wheat worldwide. The resistance gene Sbwm1 provides the only practical means of controlling this disease. Although this important gene has provided durable resistance for over thirty years, nothing is known of its structure or function. We conducted a fine-mapping study and narrowed the Sbwm1 gene region to a short chromosome segment carrying just 19 genes. This work provides a firm basis for future efforts to clone the Sbwm1 gene. The two closely flanking DNA markers were discovered for Sbwm1 that should be useful for marker-assisted selection of Sbwm1 by wheat breeders.

Technical Abstract: Soil-borne wheat mosaic virus (SBWMV) causes a serious viral disease that can significantly reduce grain yield in winter wheat worldwide. Using resistant cultivars is the only feasible strategy to reduce the losses caused by SBWMV. To fine map the resistance gene Sbwm1, 205 wheat accessions was genotyped using wheat Infinium iSelect Beadchips with 90 K SNPs. Association analysis identified 35 SNPs in 12 wheat genes and one intergenic SNP in the Sbwm1 region that showed a significant association with SBWMV resistance. Those SNPs were converted into Kompetitive Allele-Specific Polymerase assays (KASP) and analyzed in two F6-derived recombinant inbred line (RIL) populations derived from the crosses between two resistant cultivars ‘Wesley’ and ‘Deliver’ and a susceptible line ‘OK03825-5403-6’. Linkage analysis mapped this gene on chromosome 5D at intervals of 5.1 cM and 3.4 cM in the two populations, respectively. The two flanking markers in both populations delimited the gene to a 620 kb region where 19 genes were annotated. Comparative analysis identified a syntenic region of 660 kb in Ae. tauschii with 18 annotated genes and a syntenic region in chromosome 1 of B. distachyon. The candidate region includes several disease resistance related genes and we identified a PTI1-like tyrosine-protein kinase 1 gene as a putative candidate gene for Sbwm1. The two flanking SNPs for Sbwm1 can effectively separate the resistant and susceptible lines in a new diversity panel of 159 wheat germplasm. The results from this study lay a solid foundation for the cloning, functional characterization and marker-assisted selection of Sbwm1.