A diploid interspecific recombinant inbred line population for genetic mapping in potato.

Authors: ENDELMAN, JEFFREY - University Of Wisconsin; Jansky, Shelley

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/9/2016
Publication Date: 2/5/2016
Citation: Endelman, J., Jansky, S.H. 2016. A diploid interspecific recombinant inbred line population for genetic mapping in potato.. Journal of Theoretical and Applied Genetics. (2016) 129:935–943. https://doi.org/10.1007/s00122-016-2673-7.
DOI: https://doi.org/10.1007/s00122-016-2673-7

Interpretive Summary: In potato, the identification and mapping of genes important for breeding is hindered by the complex genetic makeup of the crop. Consequently, breeders have few options to select for traits based on genetic markers. In other crops, genetic analysis has been improved substantially through the use of populations designed for genetic mapping. One such type of population, a set of recombinant inbred lines (RILs), has proven to be a powerful tool to identify genes important for crop improvement. Until recently, the genetic resources have not been available to create RILs in potato. However, as a result of efforts to convert potato into a diploid hybrid crop, we have generated germplasm that can be used to create RILs. We have crossed a diploid inbred cultivated potato with an inbred wild one to create an interspecific hybrid. That hybrid was self pollinated and its offspring were carried through additional generations of self pollination to create a set of inbred lines, each of which carries a unique combination of wild and cultivated genetic regions. We are making this population available through the US Potato Genebank so it can be used to identify genes that control important tuber and plant traits. This will lead to more directed improvements through breeding.

Technical Abstract: Recombinant inbred line (RIL) populations have served as powerful mapping resources in many crop plants. In most regions of the world, potato is a tetraploid outcrossing crop. Consequently, mapping populations such as RILs have not been available. In recent years, the breeding community has been exploring the possibility of converting potato into a diploid inbred-hybrid crop. Using germplasm important to this effort, we crossed the doubled monoploid cultivated clone DM 1-3 with the inbred wild potato M6, which is homozygous for a dominant self-incompatibility inhibitor, to create an F1 hybrid. The F1 was self- pollinated to generate a phenotypically diverse F2 population, which was selfed by single-seed-descent to create F6 inbred lines (RILs). In this paper, we report on the development and characterization of the RIL population.