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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #378422
Research Project: Characterization of Quality and Marketability of Western U.S. Wheat Genotypes and Phenotypes

Location: Wheat Health, Genetics, and Quality Research

Title: The Vromindolines of cv. Hayden oat (Avena sativa L.) – A review of the Poeae and Triticeae Indolines and a suggested system for harmonization of nomenclature

Author
item Morris, Craig
item Luna, Janet
item CAFFE-TREML, MELANIE - South Dakota State University

Submitted to: Journal of Cereal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/24/2020
Publication Date: 12/11/2020
Publication URL: https://handle.nal.usda.gov/10113/7194604
Citation: Morris, C.F., Luna, J., Caffe-Treml, M. 2021. The Vromindolines of cv. Hayden oat (Avena sativa L.) – A review of the Poeae and Triticeae Indolines and a suggested system for harmonization of nomenclature. Journal of Cereal Science. 97. Article 103135. https://doi.org/10.1016/j.jcs.2020.103135.
DOI: https://doi.org/10.1016/j.jcs.2020.103135

Interpretive Summary: The Indolines are a unique group of plant proteins. Their widespread occurrence throughout the plant kingdom, and more specifically the Poaceae suggests an ancient origin with essential function(s). Typically, Indolines are ca. 450 nt with no indels. The in silico and cloning analysis presented here indicates broadly up to nine gene groups based on phylogenetic clades. Within the Poeae and Triticeae there was strong support for seven gene classes: Indoline a (Avenoindoline a, Hordoindoline a, Puroindoline a, and Secaloindoline a), Indoline b (Avenoindoline b, Hordoindoline b, Puroindoline b and Secaloindoline b), Indoline b2 (Puroindoline b2), Grain softness protein-1, and the three Vromindoline paralogs, Vin-1, Vin-2 and Vin-3. Based on these results, we suggest a system for the harmonization of the indoline nomenclature. A complete set of Vromindoine genes were isolated and sequenced from cv. Hayden oat and used to support a phylogenetic analysis of the Indolines of the Poeae and Triticeae. A system for the harmonization of the Indoline nomenclature is suggested.

Technical Abstract: The Indolines of the Poeae and Triticeae are economically important as they control seed hardness (endosperm texture) in wheat and other cereals. Here an in silico analysis of Indolines was performed at the nucleotide and protein levels across the Avena, Aegilops, Brachypodium, Hordeum, Oryza, Sorghum, Secale, and Triticum taxa. In addition, the Vromindolines from cv. Hayden oat were isolated, cloned and sequenced. The paralogous Vromindolines Vin-1, Vin-2 and Vin-3 represent three of seven Indoline gene classes: Indoline a (Avenoindoline a, Hordoindoline a, Puroindoline a, and Secaloindoline a), Indoline b (Avenoindoline b, Hordoindoline b, Puroindoline b and Secaloindoline b), Indoline b2 (Puroindoline b2), Grain softness protein-1, and the three Vromindolines. Eight unique Vromindoline genes are present in A. sativa with two or three paralogs present in each sub-genome. The other known Indolines of oat are Avenoindoline a and b, and Grain softness protein-1. Based on these results and phylogenetic analysis, we suggest a system for the harmonization of the Indoline gene nomenclature.