Complete genome sequence of three Mycoplasma gallisepticum 6/85-like isolates

Authors: Leigh, Spencer; Evans, Jeffrey - Jeff

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/14/2022
Publication Date: 8/2/2022
Citation: Leigh, S.A., Evans, J.D. 2022. Complete genome sequence of three Mycoplasma gallisepticum 6/85-like isolates. Microbiology Resource Announcements. 11(8). https://doi.org/10.1128/mra.00244-22.
DOI: https://doi.org/10.1128/mra.00244-22

Interpretive Summary: Multiple virulent Mycoplasma gallisepticum strains with genetic markers indistinguishable from vaccine strains have been identified. However, it is unknown if these strains are vaccines strains that reverted to virulence or simply strains with similar genetic markers. To identify the differences between the virulent isolates and the vaccine strain, whole genome sequencing was performed on three virulent isolates for comparison with the vaccine strain. Results show that the virulent and vaccine strains are nearly identical, although differences do exist between all the strains. These results further support the hypothesis that live-attenuated M. gallisepticum vaccines in some cases can revert to a more virulent state. This work will help identify genes required for M. gallisepticum virulence and can be applied toward future vaccine development.

Technical Abstract: Control of Mycoplasma gallisepticum infection can be accomplished through vaccination. However, virulent field strains with genetic markers identical to vaccine strains have been identified. Here we report the sequencing of three field isolates with genetic markers identical to the M. gallisepticum 6/85 vaccine strain.