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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #379216
Research Project: Maximizing the Impact of Potato Genebank Resources: Development and Evaluation of a Wild Species Genotype Diversity Panel

Location: Vegetable Crops Research

Title: Phytophthora infestans (late blight) infection assay in a detached leaf of potato

Author
item Karki, Hari
item Halterman, Dennis

Submitted to: Bio-protocol
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/19/2021
Publication Date: 2/20/2021
Citation: Karki, H.S., Halterman, D.A. 2021. Phytophthora infestans (late blight) infection assay in a detached leaf of potato. Bio-protocol. 11(4). Article e3926. https://doi.org/10.21769/BioProtoc.3926.
DOI: https://doi.org/10.21769/BioProtoc.3926

Interpretive Summary: Late blight (LB) of potato is one of the most important potato diseases. Finding LB resistant germplasm is a crucial step in breeding LB resistant potato. Here, we report the screening of the late blight resistant potato germplasm under laboratory conditions. This method uses few leaves instead of the whole plant for the disease assay, so it saves money, space, and time for the screening assay.

Technical Abstract: Phytophthora infestans is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe an improved detached leaf assay (DLA) for conducting the virulence assay of P. infestans in potato leaves. A late blight infection assay using a potato detached leaf is a semi-high throughput assay in which hundreds of plants can be screened in a laboratory setting.