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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #380642
Research Project: Genomic and Metabolomic Approaches for Detection and Control of Fusarium, Fumonisins and Other Mycotoxins on Corn

Location: Mycotoxin Prevention and Applied Microbiology Research

Title: Pure culture and DNA sequence-based identification of fusarium from symptomatic plants and diverse substrates

Author
item O Donnell, Kerry
item LARABA, IMANE - Orise Fellow
item GEISER, DAVID - Pennsylvania State University

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 3/9/2021
Publication Date: 10/23/2021
Citation: O'Donnell, K., Laraba, I., Geiser, D.M. 2022. Pure culture and DNA sequence-based identification of fusarium from symptomatic plants and diverse substrates. In: Coleman, J., editor. Fusarium Wilt. Methods in Molecular Biology, vol 2391. New York, NY: Humana. p. 1-20.
DOI: https://doi.org/10.1007/978-1-0716-1795-3

Interpretive Summary:

Technical Abstract: Fusarium ranks as the most important group of plant pathogens, responsible for a wide range of economically destructive diseases, including vascular wilts, root, crown and stem rots. In addition, head blight and ear rot diseases are associated with the accumulation of mycotoxins in cereals. With over 300 phylogenetically distinct species, and a dearth of phenotypical characteristics, DNA sequence data in most instances is the only reliable means for obtaining an accurate species identification. Here we describe how to obtain single-spored pure cultures from symptomatic host tissue and a molecular identification by querying publicly accessible DNA sequence databases using a portion of translation elongation factor 1-a (TEF1), the largest subunit of RNA polymerase (RPB1), or the second largest subunit of RNA polymerase (RPB2).