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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #380856
Research Project: Resilient, Sustainable Production Strategies for Low-Input Environments

Location: Crops Pathology and Genetics Research

Title: Transcriptomics provides a genetic signature of vineyard site and offers insight into vintage-independent inoculated fermentation outcomes

Author
item REITER, TAYLOR - University Of California, Davis
item MONTPETIT, RACHEL - University Of California, Davis
item BYER, SHELBY - University Of California, Davis
item FRIAS, ISADORA - University Of California, Davis
item LEON, ESMERALDA - University Of California, Davis
item VIANO, ROBERT - University Of California, Davis
item MCLOUGHLIN, MICHAEL - University Of California, Davis
item HALLIGAN, THOMAS - University Of California, Davis
item HERNANDEZ, DESMON - University Of California, Davis
item FIGUEROA-BALDERAS, ROSA - University Of California, Davis
item CANTU, DARIO - University Of California, Davis
item Steenwerth, Kerri
item RUNNEBAUM, RON - University Of California, Davis
item MONTPETIT, BEN - University Of California, Davis

Submitted to: mSystems
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/16/2021
Publication Date: 4/13/2021
Citation: Reiter, T., Montpetit, R., Byer, S., Frias, I., Leon, E., Viano, R., Mcloughlin, M., Halligan, T., Hernandez, D., Figueroa-Balderas, R., Cantu, D., Steenwerth, K.L., Runnebaum, R., Montpetit, B. 2021. Transcriptomics provides a genetic signature of vineyard site and offers insight into vintage-independent inoculated fermentation outcomes. mSystems. 6(2). Article e00033-21. https://doi.org/10.1128/mSystems.00033-21.
DOI: https://doi.org/10.1128/mSystems.00033-21

Interpretive Summary:

Technical Abstract: In inoculated fermentations, both Saccharomyces cerevisiae and non-Saccharomyces organisms impact final wine chemistry. Ribosomal DNA amplicon sequencing of grape musts has demonstrated that microorganisms occur non-randomly and are associated with the vineyard of origin, further suggesting a role for the wine microbiome in shaping wine outcomes. We used ribosomal DNA amplicon sequencing of grape must and RNA sequencing of primary fermentations to profile fermentations from 15 vineyards in California and Oregon across two vintages. We find that both ribosomal DNA amplicon sequencing and RNA sequencing are predictive of vineyard site. Yet, the relative abundance of fungal organisms detected by ribosomal DNA amplicon sequencing did not correlate with RNA sequencing, suggesting that the majority of the fungi detected in must are not active during fermentation. Using RNA sequencing profiles, we also detect genetic signatures of vineyard site and region during fermentation, with nitrogen, sulfur, and metal metabolism being factors important for distinguishing site.