Effects of rearing density on developmental traits of two different biotypes of the gypsy moth, Lymantria dispar L., from China and the USA

Authors: WANG, YIMING - Beijing Forestry University; Harrison, Robert - Bob; SHI, JUAN - Beijing Forestry University

Submitted to: Insects
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/10/2021
Publication Date: 2/17/2021
Citation: Wang, Y., Harrison, R.L., Shi, J. 2021. Effects of rearing density on developmental traits of two different biotypes of the gypsy moth, Lymantria dispar L., from China and the USA. Insects. https://doi.org/10.3390/insects12020175.
DOI: https://doi.org/10.3390/insects12020175

Interpretive Summary: The gypsy moth, Lymantria dispar, is a forest pest that is grouped into two biotypes: the European gypsy moth (EGM), found in Europe and North America; and the Asian gypsy moth (AGM), found in China, Russia, Korea, and Japan. Outbreaks of this pest result in high-density populations of larvae that can cause enormous damage to trees and forests. Studies have identified an influence of larval population density on gypsy moth development, but it is not known how EGM and AGM populations differ in their response to changing larval densities. We examined the effects of varying larval density on three colonies established from one EGM population and two AGM populations. All three colonies exhibited an optimal degree of larval survival and optimal rates of pupation and adult emergence at an intermediate density of five larvae/rearing container. The duration of larval development was fastest at the same intermediate density for all three colonies. Although differences in larval development time, survival, pupation and emergence were observed among the three colonies under the conditions of our study, our findings indicate that density-dependent effects on development of different gypsy moth biotypes follow the same trends. The information in this study contributes to progress towards understanding the dynamics of gypsy moth outbreaks and improving control of gypsy moth, and will be of interest to those in academia, government, and industry who work with gypsy moths and other invasive forest pests.

Technical Abstract: Life history traits of the gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) have been observed to vary with larval population density, which can increase significantly during an outbreak of this pest. Laboratory studies on density-dependent variation in gypsy moth development have focused on single populations and were limited to comparing solitary larvae with groups of larvae reared at a single density. To evaluate how density-dependent impacts on development vary with different populations and subspecies of L. dispar, we compared the effects of rearing larvae of a European gypsy moth (L. dispar dispar L.) population from Connecticut, USA; and larvae of two populations of the Asian gypsy moth (L. dispar asiatica Vnukovskij) from Guizhou and Hebei provinces in China. Larvae were reared on artificial diet at densities of one, three, five, seven, and nine larvae per 115 mL container, and the duration of larval development, percentage of surviving larvae, and the rates of pupation and emergence were measured at each density. A two-tailed response to density variation was observed for all three populations tested, with the most rapid larval development and the highest values of survival, pupation, and emergence observed at a density of 5 larvae/container. Although differences in larval development time, survival, pupation and emergence were observed among the different populations under the conditions of our study, our findings indicate that density-dependent effects on development of different gypsy moth subspecies and populations follow the same trends.