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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Livestock Bio-Systems » Research » Publications at this Location » Publication #382143
Research Project: Improving Livestock Production by Developing Reproductive and Precision Management Technologies

Location: Livestock Bio-Systems

Title: Relationship among granulosa cell GnRH-I, GnRH-II, and GnRH-R mRNA abundance and follicular fluid steroid hormone concentrations of bovine antral follicles at specific stages of follicular development

Author
item RICH, JERICA - Arkansas State University
item NORTHROP-ALBRECHT, EMMALEE - South Dakota State University
item EPPERSON, KAITLIN - South Dakota State University
item MENEGATTI ZOCA, SAULO - South Dakota State University
item PERKINS, STEPHANIE - South Dakota State University
item DALY, RUSSELL - South Dakota State University
item Cushman, Robert - Bob
item PERRY, GEORGE - South Dakota State University

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2021
Publication Date: 11/3/2021
Citation: Rich, J.J., Northrop-Albrecht, E.J., Epperson, K.M., Menegatti Zoca, S., Perkins, S.D., Daly, R.F., Cushman, R.A., Perry, G.A. 2021. Relationship among granulosa cell GnRH-I, GnRH-II, and GnRH-R mRNA abundance and follicular fluid steroid hormone concentrations of bovine antral follicles at specific stages of follicular development [abstract]. Journal of Animal Science. 99(Supplement 3):126. https://doi.org/10.1093/jas/skab235.230.
DOI: https://doi.org/10.1093/jas/skab235.230

Interpretive Summary:

Technical Abstract: Transcript abundance of two forms of GnRH and its receptor have been characterized in bovine ovaries. The objective was to investigate the relationship of GnRH-1, GnRH-2, GnRH-R, intrafollicular estradiol (E2) and progesterone (P4) during follicular development. Ovaries were collected from beef cows at specific stages of follicular development [pre-selection (PRE, n=9), post-selection (POST, n=9), and post-selection 24h after luteal regression (PG, n=9)]. The largest follicle per stage was aspirated to obtain granulosa cells (GC) and follicular fluid (FF). Total cellular RNA was extracted from GC and RT-PCR was performed for GnRH-1, GnRH-2, GnRH-R and GAPDH. Radioimmunoassays were performed to determine FF concentrations of E2 and P4. Data were analyzed using the MIXED and REG procedures in SAS. There was no difference (P=0.23) in mRNA abundance of GnRH-2, GnRH-R or FF concentrations of P4 across follicular stages. There was an effect of stage on FF E2 (PRE: 17,925±20,273, POST: 28,458±21,503, and PG: 252,616±21,503 pg/mL; P<0.01). Stage affected GnRH-1 mRNA abundance (PRE: 2.28±0.55, POST: 0.92±0.55, and PG: 0.11±0.55; P=0.03). There was no relationship of GnRH-1 and GnRH-2 mRNA abundance or effect of FF P4 on GnRH-R mRNA abundance (P=0.23). There was no effect of FF P4 on GnRH-1 mRNA abundance (P=0.68). There was no effect of FF E2 on GnRH-2 mRNA abundance (P=0.66). As FF E2 increased GnRH-R mRNA abundance tended to increase (P=0.10;r2=0.13). As FF P4 concentrations increased GnRH-2 mRNA abundance tended to decrease (P=0.09;r2=0.12). As FF E2 increased GnRH-1 mRNA abundance decreased (P=0.02;r2=0.20). In conclusion, there were differences in peptide and receptor mRNA abundance of GnRH in relation to FF E2 and P4 at specific stages of follicular development. This is supportive of a regulatory relationship between ovarian GnRH and steroidogenesis. This work is supported by AFRI Grant No. 2018-67016-27578 from USDA.