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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #382532
Research Project: Genetic Mechanisms and Improvement of Insect Resistance in Wheat, Barley, and Sorghum

Location: Peanut and Small Grains Research Unit

Title: Characterization of PmBN418, a wheat powdery mildew resistance gene on the rye 1RS chromosome arm

Author
item Xu, Xiangyang
item Bai, Guihua
item Li, Genqiao
item Cowger, Christina
item Bernardo, Amy
item CARVER, BRETT - Oklahoma State University
item St Amand, Paul
item BIAN, RUOLIN - Kansas State University

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/7/2021
Publication Date: 9/15/2021
Citation: Xu, X., Bai, G., Li, G., Cowger, C., Bernardo, A.E., Carver, B., St Amand, P.C., Bian, R. 2021. Characterization of PmBN418, a wheat powdery mildew resistance gene on the rye 1RS chromosome arm. Crop Science. 61(6):4194-4201. https://doi.org/10.1002/csc2.20637.
DOI: https://doi.org/10.1002/csc2.20637

Interpretive Summary: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a disease of global importance, and new powdery mildew resistance genes that can be directly used in wheat breeding are urgently needed. A recombinant inbred line (RIL) population from CI 17884 × Bainong 418 was genotyped using single nucleotide polymorphism (SNP) markers, and evaluated for responses to Bgt isolates OKS(14)-B-3-1 and NEI1-3. Genetic mapping identified a powdery mildew resistance gene, designated PmBN418, on a 1BL.1RS translocation segment in Bainong 418. Given that PmBN418 conferred resistance to Bgt isolates virulent to Pm8 that resides on the chromosome arm 1RS translocated from rye, PmBN418 is either a new gene or a new allele of Pm8. Bainong 418, a high-yielding cultivar with good resistance to several Bgt isolates, is a good resistance source for wheat breeding, and marker-assisted introgression of PmBN418 into locally adapted cultivars is feasible.

Technical Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a disease of global importance, and new powdery mildew resistance genes that can be directly used in cultivar development are urgently needed. A recombinant inbred line population from 'CI 17884' × 'Bainong 418' was genotyped using single nucleotide polymorphism markers generated from genotyping-by-sequencing, and evaluated for responses to Great Plains Bgt isolates OKS(14)-B-3-1 and NEI1-3. Genetic mapping identified a powdery mildew resistance gene, designated PmBN418, on a 1BL.1RS translocation segment in Bainong 418. Given that PmBN418 conferred resistance to Bgt isolates virulent to Pm8 that resides on the chromosome arm 1RS translocated from rye (Secale cereale L.) cv. ‘Petkus’, PmBN418 is either a new gene or a new allele of Pm8. Phenotypic and genomic data analyses indicated segregation distortion of the powdery mildew resistance gene due to reduced transmission of the wheat (Triticum aestivum L.)–rye 1BL.1RS translocation segment from Bainong 418, while the wheat–Triticum speltoides translocation segment 7S#1 in CI 17884 was preferentially transmitted. Bainong 418, a high-yielding cultivar with good resistance to several U.S. Bgt isolates, is a useful resistance source for wheat breeding, and the availability of the Kompetitive Allele Specific PCR (KASP) marker KASP-1RS-1 makes marker-assisted introgression of PmBN418 into locally adapted cultivars feasible.