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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Publications at this Location » Publication #382589
Research Project: Grain Composition Traits Related to End-Use Quality and Value of Sorghum

Location: Grain Quality and Structure Research

Title: Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis Causing Pathogens

Author
item SYDNEY, SCHNUR - Kansas State University
item R.G., AMACHAWADI - Kansas State University
item GIOVANNA, BACA - Kansas State University
item SARAH, SEXTON-BOWSER - Kansas State University
item DAVINA, RHODES - Colorado State University
item Smolensky, Dmitriy
item Herald, Thomas
item RAMASAMY, PERUMAL - Kansas State University
item D. U., THOMSON - Iowa State University
item T. G., NAGARAJA - Kansas State University

Submitted to: Antibiotics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/14/2021
Publication Date: 5/17/2021
Citation: Sydney, S.E., R.G., A., Giovanna, B., Sarah, S., Davina, R., Smolensky, D., Herald, T.J., Ramasamy, P., D. U., T., T. G., N. 2021. Antimicrobial activity of sorghum phenolic extract on bovine foodborne and mastitis causing pathogens. Antibiotics. https://doi.org/10.3390/antibiotics10050594.
DOI: https://doi.org/10.3390/antibiotics10050594

Interpretive Summary: Bovine mastitis is a disease that afflicts dairy cattle and has an adverse animal health and economic cost. The goal of this study was to test the efficacy of sorghum polyphenols against 8 bacteria species known to cause mastitis in cattle. Sorghum polyphenols were found to effectively inhibit the grown of 4 of the 8 bacterial species. These results suggest that further research is needed to explore the application of high polyphenol sorghum in preventing bovine mastitis.

Technical Abstract: Antimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds and the grain extract may have the potential as a natural antimicrobial alternative. The study’s objective was to determine antimicrobial effects of sorghum phenolic extract on bacterial pathogens that cause bovine mastitis and human foodborne illnesses. Bacterial pathogens tested included Escherichia coli, Salmonella Typhimurium, Campylobacter jejuni, Campylobacter coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Klebsiella oxytoca, Staphylococcus aureus, and Enterococcus faecalis. Antibacterial activities of sorghum phenolic extracts were determined by agar well diffusion assay. Sorghum phenolic extract was added to the wells in concentrations of 0, 100, 200, 500, 1,000, or 4,000 µg/ mL. Plates were incubated for 18-24 hours and the diameter of each zone of inhibition was measured. The results indicated that sorghum phenolic extract had inhibitory effects on Staphylococcus aureus, Enterococcus faecalis, Campylobacter jejuni, and Campylobacter coli.