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Research Project: Monitoring and Molecular Characterization of Antimicrobial Resistance in Foodborne Bacteria

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Title: Insights into CTX-producing-Escherichia coli clones from humans and companion animals from Egypt

Author
item RAMADAN, HAZEM - Mansoura University
item Hiott, Lari
item Barrett, John
item AHMED, MARWA - Mansoura University
item Woodley, Tiffanie
item Frye, Jonathan
item Jackson, Charlene

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/14/2021
Publication Date: 6/20/2021
Citation: Ramadan, H., Hiott, L.M., Barrett, J.B., Ahmed, M., Woodley, T.A., Frye, J.G., Jackson, C.R. 2021. Insights into CTX-producing-Escherichia coli clones from humans and companion animals from Egypt. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Companion animals have substantially contributed to the transmission of several zoonotic infections to humans, yet their potential role for the dissemination of antimicrobial resistance, particularly plasmid mediated genes, to humans has been reportedly underestimated especially in developing countries. Herein, a set of 65 CTX-producing-E. coli isolates from humans (n=33) and companion animals (n=32), collected over 8 months from December 2015 to August 2016 in Mansoura, Egypt was examined for clonal diversity using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The human E. coli isolates (n=33) in this study were recovered from mid-stream urine samples of outpatients (n=23) attending Mansoura University Specialized Internal Medicine Hospital and healthy persons (n=10). Companion animal E. coli were isolated from household dogs (n=24) and cats (n=8) admitted to private pet clinics in the same district from which human samples were taken. Antimicrobial resistance phenotypes and genotypes as well as plasmid replicon types were also determined. PFGE clustered isolates from both sources into seven clusters with =75% similarity. Based on MLST results, 20 different sequence types (STs) were distinguished. Approximately, two third of the isolates were designated into four STs: ST167 (13/65, 20%), ST457 (11/65, 16.9%), ST69 (10/65, 15.4%) and ST48 (9/65, 13.8%). The most predominant STs among human E. coli were ST167 (12/33, 36.4%) and ST69 (10/33, 30.3%), while ST457 (11/32, 34.4%) and ST48 (9/32; 28.1%) were the prevalent types in isolates from dogs and cats. Although the majority of STs were identified from one host, either human or companion animals, two STs (ST167 and ST648) were identified from both sources. The circulation of similar STs in CTX-producing E. coli from humans and companion animals reveals a possible epidemiological link that warrants further investigation with whole genome-based phylogeny.