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ARS Home » Southeast Area » Tifton, Georgia » Crop Genetics and Breeding Research » Research » Publications at this Location » Publication #382986
Research Project: Improvement of Genetic Resistance to Multiple Biotic and Abiotic Stresses in Peanut

Location: Crop Genetics and Breeding Research

Title: Comparative transcriptome analysis identified candidate genes for late leaf spot resistance and cause of defoliation in groundnut

Author
item GANGURDE, SUNIL - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item NAYAK, SPURTHI - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item JOSHI, PUSHPESH - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item SHILP, PUROHIT - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item SUDINI, HARI - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item CHITIKINENI, ANNAPURNA - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item HONG, YANBIN - GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES
item Guo, Baozhu
item CHEN, X - GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES
item PANDEY, M - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item VARSHNEY, R - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/22/2021
Publication Date: 4/26/2021
Citation: Gangurde, S.S., Nayak, S.N., Joshi, P., Shilp, P., Sudini, H.K., Chitikineni, A., Hong, Y., Guo, B., Chen, X., Pandey, M., Varshney, R.K. 2021. Comparative transcriptome analysis identified candidate genes for late leaf spot resistance and cause of defoliation in groundnut. International Journal of Molecular Sciences. 22, 4491:1-23.

Interpretive Summary: Despite having information for peanut genome regions associated markers or QTLs, there is no information on the candidate genes associated with leaf spot disease resistance in peanut. In this study, we conducted comparative transcriptome analysis to study the expression of genes in reported QTL regions in resistant and susceptible peanut lines. The comparatives study identified important differentially expressed genes at different disease development stages including defoliation. Important disease resistance genes such as RPP13-like and NBS-LRR genes in previously reported QTL regions were identified for leaf spot resistance. Pathway analysis identified important pathways such as antibiotic biosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis which were triggered in both A and B sub-genomes. Ethylene responsive factors were also identified, which were highly expressed in susceptible genotypes at the defoliation stage in susceptible genotypes. The identified disease resistance genes can be deployed in marker-assisted breeding for improvement of late leaf spot resistance in peanut.

Technical Abstract: Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression line (ICGV 13208), and identified a total of 12,164 and 9954 DEGs (differentially expressed genes) respectively in A- and B- subgenomes of tetraploid groundnut. There were 135 and 136 unique pathways triggered in A- and B-subgenomes respectively upon N. personata infection. Highly upregulated putative disease resistance genes, a RPP-13-like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB) were identified on chromosome A02 and A03, respectively for LLS resistance. Mildew resistance Locus (MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce the yield loss in groundnut.