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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #383311
Research Project: Genetic Enhancement of Seed Quality and Plant Health Traits, and Designing Soybeans with Improved Functionality

Location: Crop Production and Pest Control Research

Title: Identification and molecular mapping of Rps14, a gene conferring broad-spectrum resistance to Phytophthora sojae in soybean

Author
item CHEN, LIYANG - Purdue University
item WANG, WEIDONG - Purdue University
item PING, JEIQING - Purdue University
item FITZGERALD, JOSHUA - Purdue University
item Cai, Guohong
item CLARK, CHANCELOR - Purdue University
item AGGARWAL, RAJAT - Corteva Agriscience
item MA, JIANXIN - Purdue University

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/2/2021
Publication Date: 8/9/2021
Citation: Chen, L., Wang, W., Ping, J., Fitzgerald, J.C., Cai, G., Clark, C., Aggarwal, R., Ma, J. 2021. Identification and molecular mapping of Rps14, a gene conferring broad-spectrum resistance to Phytophthora sojae in soybean. Journal of Theoretical and Applied Genetics. https://doi.org/10.1007/s00122-021-03933-9.
DOI: https://doi.org/10.1007/s00122-021-03933-9

Interpretive Summary: Phytophthora root and stem rot is one of the most devastating soybean diseases in the United States. It was estimated to cause economic loss of $7.4 billion between 1996 and 2016. Planting soybean varieties carrying Rps genes that confer resistance to the causal pathogen, Phytophthora sojae, is the most economical and effective way to manage this disease. The pathogen can evolve and overcome deployed Rps genes. As such, it is important to find and deploy new Rps genes. In this study, we characterize a new gene in the soybean land race. It confers broad spectrum resistance to Phytophthora sojae. This study provides another tool in the ongoing effort to managing Phytophthora root and stem rot of soybean.

Technical Abstract: Phytophthora sojae is the causative agent for Phytophthora root and stem rot in soybean [Glycine max (L.) Merr.] and can be managed by deployment of resistance to P. sojae (Rps) genes. PI 340,029 is a soybean landrace carrying broad- spectrum resistance to the pathogen. Analysis of an F2 population derived from a cross between PI 340,029 and a susceptible cultivar ‘Williams’ reveals that the resistance to P. sojae race 1 is conferred by a single gene, designated Rps14, which was initially mapped to a 4.5-cM region on the short arm of chromosome 3 by bulked segregant analysis (BSA), and subsequently narrowed to a 1.48 cM region corresponding to 229-kb in the Williams 82 reference genome (Wm82 v2.a1), using F3:4 fami- lies derived from the F2 population. Further analysis indicates that the broad-spectrum resistance carried by PI 340,029 is fully attributable to Rps14. The genomic sequences corresponding to the defined Rps14 region from a set of diverse soybean varieties exhibit drastic NBS-LRR gene copy number variation, ranging from 3 to 17 copies. Ultimate isolation of Rps14 would be critical for precise selection and deployment of the gene for soybean protection.