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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Citrus and Other Subtropical Products Research » Research » Publications at this Location » Publication #383835
Research Project: Quality, Shelf-life and Health Benefits for Fresh, Fresh-cut and Processed Products for Citrus and Other Tropical/Subtropical-grown Fruits and Vegetables

Location: Citrus and Other Subtropical Products Research

Title: Neohesperidin Dihydrochalcone and Neohesperidin Dihydrochalcone-O-Glycoside Attenuate Subcutaneous Fat and Lipid Accumulation by Regulating PI3K/AKT/mTOR Pathway In Vivo and In Vitro

Author
item KWAN, MINSEO - Ewha Woman'S University
item KIM, YERIN - Ewha Woman'S University
item LEE, JINYE - Ewha Woman'S University
item Manthey, John
item KIM, YANG - Seoul National University
item KIM, YURI - Ewha Woman'S University

Submitted to: Nutrients
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/23/2022
Publication Date: 3/4/2023
Citation: Kwan, M., Kim, Y., Lee, J., Manthey, J.A., Kim, Y., Kim, Y. 2023. Neohesperidin Dihydrochalcone and Neohesperidin Dihydrochalcone-O-Glycoside Attenuate Subcutaneous Fat and Lipid Accumulation by Regulating PI3K/AKT/mTOR Pathway In Vivo and In Vitro. Nutrients. 14/1087. https://doi.org/10.3390/nu14051087.
DOI: https://doi.org/10.3390/nu14051087

Interpretive Summary: Neohesperidin dihydrochalcone, a semi-natural compound from bitter orange, is an intense sweetener. The anti-obesity effects of NHDC and its glycosidic compound, neohesperidin dihydrochalcone-O-glycoside , were investigated. Positive anti-obesity effects were found to occur in experimental mouse trials. This opens new applications for these sweetener compounds, and for the citrus compounds that are the starting materials for neohesperidin dihydrochalcone manufacture.

Technical Abstract: Neohesperidin dihydrochalcone (NHDC), a semi-natural compound from bitter orange, is an intense sweetener. The anti-obesity effects of NHDC and its glycosidic compound, NHDC-O-glycoside (GNHDC), were investigated. C57BLKS/J db/db mice were supplemented with NHDC or GNHDC (100 mg/kg b.w.) for 4 weeks. Body weight gain, subcutaneous, and total adipose tissues were decreased in the NHDC and the GNHDC groups. Fatty acid uptake, lipogenesis and adipogenesis-related genes were decreased, whereas ß-oxidation and fat browning-related genes were up-regulated in the sweetener groups. Furthermore, both sweeteners suppressed the level of triglyceride accumulation, lipogenesis, adipogenesis, and proinflammatory cytokines in the 3T3-L1 cells. The PI3K/AKT/mTOR pathway was also down-regulated and AMPK was phosphorylated in the treatment groups. These results suggested that NHDC and GNHDC exerted anti-obesity effects by regulating the PI3K/AKT/mTOR pathway and AMPK-related lipogenesis and fat browning.