Genetic diversity of MHC-BF/BL region in 21 chicken populations

Authors: YUAN, YIMING - China Agriculture University; Zhang, Huanmin; YI, GUOQIANG - Chinese Academy Of Agricultural Sciences; YOU, ZHEN - China Agriculture University; ZHAO, CHUNFANG - China Agriculture University; YUAN, HAIXU - China Agriculture University; WANG, KEJUN - Henan University Of Technology; LI, JUNYING - China Agriculture University; YANG, NING - China Agriculture University; LIAN, LING - China Agriculture University

Submitted to: Frontiers in Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/26/2021
Publication Date: 8/13/2021
Citation: Yuan, Y., Zhang, H., Yi, G., You, Z., Zhao, C., Yuan, H., Wang, K., Li, J., Yang, N., Lian, L. 2021. Genetic diversity of MHC-BF/BL region in 21 chicken populations. Frontiers in Genetics. 12:710770. https://doi.org/10.3389/fgene.2021.710770.
DOI: https://doi.org/10.3389/fgene.2021.710770

Interpretive Summary: The Chicken genome contains a special region known as the major histocompatibility complex (MHC), which is composed of at least three regions referred to as BL, BF and BG. The MHC is known for being associated with immunity and disease resistance. The diversity of the chicken MHC was thoroughly investigated in 21 Chinese indigenous chicken lines as well as two highly inbred White Leghorns lines known as lines 63 and 72. A large number of genetic variants were identified within the MHC BF and BL region and more than half of the variants were novel variants. These findings not only dramatically expand the current genetic database, but also provide high-quality molecular markers potentially implementable in poultry breeding programs.

Technical Abstract: The chicken major histocompatibility complex (MHC) on chromosome 16 is the most polymorphic region across the whole genome, which is also an ideal model system for genetic diversity investigation. The MHC BF/BL region is 92 kb in length with high GC content consisting of 18 genes and 1 pseudogene (Blec4), which plays important roles in immune response. To evaluate polymorphism of the Chinese indigenous chickens as well as to analyze the effect of selection to genetic diversity, we used WaferGen platform to identify sequence variants of the BF/BL region in 21 chicken populations, including the Red Jungle Fowl, 16 Chinese domestic breeds, two introduced breeds, Cornish, White Leghorns , and two well-known Marek’s disease resistance/susceptible White Leghorn lines 63 and 72. A total of 6,170 single nucleotide polymorphisms (SNPs) and 595 insertions and deletions (INDELs) in the BF/BL region were identified among the 21 populations, of which 4,040 SNPs (65%) and 565 INDELs (95%) were novel. Most of the variants were within the intron and the flanking regions. The average variation density was 65 SNPs and 5.8 INDELs per kb, indicating dramatically high diversity of this region. Furthermore, BF2 was identified as the hypervariable genes with 148 SNPs per kb. The Chinese domestic populations showed higher diversity than the White Leghorns and Cornish. The indigenous breeds, Naodan Yao, Gushi, and Xishuangbanna Game chickens, were the top three with the highest density of SNPs and INDELs. The highly inbred lines 63 and 72 have the lowest diversity, which might be resulted from a long-term intensity selection exerted for decades. Collectively, we refined the genetic map of chicken MHC-BF/BL region and evaluated genetic diversity of 21 chicken populations. Abundant genetic variants were identified, which not only dramatically expanded the current Ensembl SNP database, but also provided high-quality molecular markers for breeding.