Evidence for glandless cottonseed kernel extract down-regulating gene expression in human colon cancer cells

Authors: Cao, Heping; Sethumadhavan, Kandan; WU, XIAOYU - Jiangxi Agricultural University; ZENG, XIAOCHUN - Yichun University; ZHANG, LIN - Central South University Of Forestry And Technology

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/18/2021
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cotton plant provides economically important fiber and cottonseed. Cottonseed contributes to approximately 20% of the crop value, but its value could be increased by providing high value bioactive compounds and polyphenolic extracts aimed at improving nutrition and preventing diseases. Cottonseed is either glanded or glandless depending on gossypol glands. Glandless seeds contain very low amount of gossypol and are generally considered safe to use. The objective of this study was to test the hypothesis if glandless cottonseed extract could regulate gene expression in human colon cancer cells. COLO 225 cells were treated with ethanol extract followed by evaluating cell viability and gene expression with MTT and qPCR assays. Glandless seed kernel extract showed minor effect on cell viability. qPCR method was used to analyze 55 mRNA levels involved in several pathways including DGAT, GLUT, TTP, IL, gossypol-regulated genes and TTP-mediated mRNAs. Glandless seed kernel extract significantly reduced mRNA levels of several key genes involved in glucose transport, lipid biosynthesis and inflammation. The inhibitory effects of glandless seed kernel extract on gene expression may provide an opportunity for improving nutrition and health. This may increase glandless cottonseed value by using ethanol extract as a nutritional supplement and health intervention agent.