Skip to main content
ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #385659
Research Project: Development of New Production Methodologies for Biocontrol Agents and Fastidious Microbes to Improve Plant Disease Management

Location: Crop Bioprotection Research

Title: Functional annotation unravels probiotic properties of a poultry isolate, Bacillus velezensis CGS1.1

Author
item SONI, RITESHRI - Sardar Patel University
item KEHARIA, HARESHKUMAR - Sardar Patel University
item Dunlap, Christopher
item PANDIT, NINAD - Zytex Biotech Pvt Ltd
item DOSHI, JAYRAJ - Zytex Biotech Pvt Ltd

Submitted to: LWT - Food Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/14/2021
Publication Date: 9/15/2021
Publication URL: https://handle.nal.usda.gov/10113/7505877
Citation: Soni, R., Keharia, H., Dunlap, C.A., Pandit, N., Doshi, J. 2022. Functional annotation unravels probiotic properties of a poultry isolate, Bacillus velezensis CGS1.1. LWT - Food Science and Technology. 153. Article 112471. https://doi.org/10.1016/j.lwt.2021.112471.
DOI: https://doi.org/10.1016/j.lwt.2021.112471

Interpretive Summary: An ARS researcher from Peoria, Illinois, collaborated with scientists in India to characterize a novel bacterial isolate of Bacillus from poultry feces. Strains of Bacillus have been successfully commercialized as probiotics for use in poultry, livestock, and aquaculture feeds. The goal of this project was to isolate strains of Bacillus from poultry, which were antagonistic to known poultry pathogens. One strain was identified and showed high activity to inhibit the pathogen while being able to tolerate the physiological stresses needed to be a probiotic. Future studies will explore the commercial potential of the strain as poultry feed additive.

Technical Abstract: Probiotics are an eco-friendly and prospective substitute for antibiotic growth promoters. The present investigation aimed to dissect the probiotic attributes of a poultry isolate CGS1.1 by integrating genomics and phenotypic traits. The strain exhibited bile salt hydrolase activity and ~73% survival in simulated gastrointestinal juices. It displayed notable aggregation, cell surface hydrophobicity, biofilm formation, and antimicrobial activity against gram-positive and gram-negative bacterial pathogens. Interestingly, the strain CGS1.1 completely inhibited the growth of E. coli and S. enterica after 24 h in liquid co-culture assay. The strain CGS1.1 effectively hydrolyzes starch, cellulose, pectin, tributyrin, casein, and phytate. The in silico genome analysis revealed its identity as Bacillus velezensis. Genome annotation revealed the presence of genes encoding the aforementioned probiotic properties. Furthermore, CGS1.1 was found to be non-hemolytic and sensitive to major classes of antibiotics. No potent virulent factor or toxin encoding genes could be predicted in CGS1.1 genome signifying its safety. The results demonstrate the potential of CGS1.1 as a probiotic to promote chicken growth and control pathogens.