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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #385845
Research Project: Introgression of Novel Resistant Genes and Development of Integrated Production Systems for Managing Reniform Nematodes in Cotton

Location: Crop Genetics Research

Title: Cotton infection by Rotylenchulus reniformis as determined from four root system portions

Author
item Stetina, Salliana - Sally
item Erpelding, John

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 9/14/2021
Publication Date: 10/27/2021
Citation: Stetina, S.R., Erpelding, J.E. 2021. Cotton infection by Rotylenchulus reniformis as determined from four root system portions. Journal of Nematology. 53:34. https://doi.org/10.21307/jofnem-2021-095.
DOI: https://doi.org/10.21307/jofnem-2021-095

Interpretive Summary:

Technical Abstract: Rapid screening based on the number of females infecting the cotton root system is typically destructive to the plant, although recovery of resistant plants is desired by breeders. The objective of this work was to determine whether resistance designations could be determined reliably if only a portion of the root system was examined. In a repeated growth chamber test, three susceptible cotton lines (Gossypium hirsutum ‘Deltapine 16’ and MD 25 and G. arboreum A2-101) and three resistant cotton lines (G. hirsutum LONREN 2, G. arboreum A2-190, and G. barbadense TX 110) were inoculated 5 to 7 days after planting with 1,000 vermiform reniform nematodes (Rotylenchulus reniformis) suspended in water. Four weeks later, the plants were removed from the pots and the roots were gently rinsed free of soil. Four root retention treatments were examined. The root system was cut off at either 0, 1, 2.5, or 5 cm below the soil line and used for counting the number of females that had infected the roots. The test was a completely randomized design with a factorial treatment arrangement (genotype x root retention) and 5 replications. Data from both trials were combined and transformed [log10(x+1)] prior to analysis of variance. Significant differences were found between genotypes (F=113.55, P<0.0001) with respect to number of females, with each genotype displaying resistance or susceptibility as expected: Deltapine 16 = 113.5, A2-101=58.4, MD 25=57.4, TX 110=19.2, A2-190=8.4, and LONREN 2=7.9. The number of females did not differ significantly based on the amount of root tissue retained with the stem (F=2.50, P=0.0609), and no significant interactions between genotype and root retention (F=1.40, P=0.1504) were found. Thus, resistance or susceptibility could be determined reliably across a range of cotton genotypes while keeping some root tissue with the plant to facilitate recovery of desirable individuals.